Abstract:

Objective    To evaluate the effects of the CO₂-independent cell culture system on the proliferation of bladder cancer cells T-24. Methods       Bladder cancer cells T-24 were cultured in the CO₂-independent cell culture system at 37℃， in a 5% carbon dioxide incubator (CO₂-B group) and in an air incubator(air-B group)， and in the CO₂dependent cell culture system at 37℃, in a 5% carbon dioxide incubator(CO₂-A group) and in an air incubator(air-A group). The pH values of these two cell culture mediums were measured for 7 consecutive days. Differences of the cell viability were assayed with MTS and the colony forming assay. The cell cycle and apoptosis ratio of T-24 cells treated in the two cell culture systems were tested by FCM. Expressions of survivin mRNA and caspase-3 mRNA in T-24 cells cultured with the two systems were detected by RT-PCR, while expressions of survivin and caspase-3 proteins were detected by Western blot. Results    The pH trends of the two cell culture mediums were the same. T-24 cell proliferation and expressions of survivin and caspase-3 mRNAs and proteins had no obvious difference. Conclusion    The CO₂-independent cell culture system is not obviously different from the CO₂-dependent cell culture system in cell proliferation and gene expression.

Key words: T-24 bladder cancer； CO₂-independent cells；Survivin gene；Caspase-3 protein