Abstract:

Objective   To explore the effect of total panax notoginsenosides (TPNS) on expression of cell differentiation antigen 40 (CD40) and vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) induced by oxidized low density lipoprotein (oxLDL). Methods    Primary culture HUVECs cells were divided into five groups: the control, the model, the low dose (100mg/L) the TPNS, high dose (200mg/L) TPNS and the Simvastain group. Apart from the control group, cells in other groups were given corresponding treatment. Cytoactivity was measured by MTT.  mRNA expression of VCAM-1 was detected by realtime quantitative RT-PCR. Protein expression of CD40 was measured by Western blot. Results    TPNS and Simvastatin could up-regulate the cytoactivity of HUVECs(P＜0.05, P＜0.001),  and down-regulate expression of CD40(P＜0.05, P＜0.01), VCAM-1（P＜0.001）in HUVECs induced by oxLDL. In addition, the effect of high dose TPNS was superior to the low dose TPNS (P＜0.05). Conclusion    TPNS can relieve injury and lower expression of immune inflammatory factors in HUVECs induced by oxLDL. TPNS has a potential effect in preventing and treating atherosclerosis. 

Key words: Total panax notoginsenosides; Oxidized low density lipoprotein; Human umbilical vein endothelial cells; Cell differentiation antigen 40; Vascular cell adhesion molecule-1