Abstract:

Objective  To study the inhibitory mechanism of polypeptide extract from scorpion venom(PESV) on the growth and metastasis of Lewis lung carcinomas(LLC). Methods  Thirty C57BL/6J mice were subcutaneously inoculated with LLC cells suspension(1×10-7/mL) in the right armpit. The tumor-bearing mice were randomlydivided into three groups: the control group, the 5-fluorouracil(5-FU)group and the PESV group. PESV was intragastrically subjected to the mice for 18 days. The tumor volume and tumor inhibitory rate were determined. A LLC cell suspension(5×10-6/mL) was injected into the tail veins of another twenty C57BL/6J mice, which were randomly divided into two groups: the control group and the PESV group. PESV was intragastrically subjected to the mice for 28 days. The mice were killed and the numbers of lung metastatic nodules were measured. Expressions of OPN and MMP-9 were determined by immunohistochemical-staining and ELISA..Results  The growth and metastasis inhibitory rate of PESV were respectively 46.02% and 76.19%. Expression levels of OPN and MMP-9 were both decreased in the tumor and serum． Compared with the control group, the differences were both significant(P＜0.05). Conclusion  PESV has an inhibitory effect on growth and metastasis of Lewis lung carcinoma.

Key words: Scorpion venoms; Lung neoplasms; Gelatinase B; Mice;Osteopontin