Abstract:

Objective  To research the effect of the Naoshenkang capsule on expressionof hypoxia-inducible factor-1 alpha of oxidative damaged rat hippocampal neuronsin high glucose. [JP2]Methods  The primarily cultured neonate rat hippocampal neurons were randomly divided into eight groups: the control group, the high glucose group, the xanthine/xanthine oxidase group(X/XO group), the ligustrazine group, the normal sodium group and the small, middle, and large dosage of Naoshenkang groups. The control group was raised in 5.5mmol/L DMEM, and the other groups treated by xanthine/xanthine oxidase except that the high glucose group was raised in 25mmol/L DMEM. Themethod of Real-Time PCR was used to detect mRNA expression of HIF-1α. Results  Expression of HIF-lα mRNA of the high glucose group was significantly higher than that of the control group (P＜0.01), but lower than that of the X/XO group (P＜0.05). Expression of HIF-lα mRNA of the ligustrazine group and all Chinese Medicine groups were significantly lower than that of the X/XO group, and the middle dosage of Chinese Medicine groups was the lowest (P＜0.01).Conclusions  High glucose can cause injury of hippocampal neurons and the Naoshenkang capsule has an inhibitory effect on it. Its mechanism may be that the Chinese Medicine can inhibit expression of HIF-lα mRNA and improve tissue hypoxia.

Key words: Hippocampal neurons; Naoshenkang capsule; High glucose; Xanthine/xanthine oxidase; Hypoxia-inducible factor-1 alpha; Rat, wistar