Abstract:

Objective  To explore the biocompatibility between neuroepithelial stem cells (NEPs) and chitosan fibers, and to observe whether the NEP stem cells are able to proliferate and differentiate on chitosan fibers. Methods  Neural-tube-derived NEPs were seeded on chitosan fibers in vitro. MTT assay was used to determine the biocompatibility of chitosan fibers. A light and scanning electronic microscope was performed to examine the growth and survival. Immunocytochemical staining was used to examine the differentiation of NEPs. Results  NEPs survived well on the surface of chitosan fibers, furthermore, they firmly attached to the chitosan fibers and differentiated into neurons and glia.  Conclusion  Chitosan fibers have excellent biocompatibility with NEPs, which offers a possible substitute for repairing peripheral nerve injury.

Key words: Stem cells;  Chitosan fiber; Rats, Wistar