JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2009, Vol. 47 ›› Issue (9): 58-61.

• Articles • Previous Articles     Next Articles

Effect of  glutathione on proliferation of KLE and 
HEC1b endometrial cancer cells

ZHANG Peihai1,　DING Ning1,　KONG Feng2,　BI Wenxiang2   

  1. 1. Department of Obstetrics and Gynecology, Qilu Hospital of Shangdo ng University, Jinan 250012, China；
    2. Institute of Biochemistry and Molacular Biology, School  of Medicine, Shandong University, Jinan 250012, China
  • Received:2009-02-22 Published:2009-09-16

PDF (PC)

195

Abstract:

To investigate the effects of glutathione on proliferation of typeⅠand Ⅱ endometrium cancer cells and to explore its related mechanism. Method① TypeⅠand Ⅱ endometrium cancer cells were challenged by GSH, and proliferation of cells was observed with MTT assay. ② TypeⅠor Ⅱ endometrium cancer cells were treated with 5?mmol/L GSH and the cellular ROS, GSH, and GSSG levels and SOD, CAT and GPx activities were determined. ResultsWithout treatment with GSH, typeⅠ endometrium cancer cells, compared with type Ⅱ endometrium cancer cells, had a lower ROS level and CAT activity and higher GSH level, GSH/GSSG ratio, and SOD and GPx activities. In the condition of treating the cells with reductive GSH, proliferation was promoted in typeⅠendometrium cancer cells while it was inhibited in typeⅡendometrium cancer cells. The ROS level was decreased in both typeⅠand Ⅱ endometrium cancer cells. The GSH level, GSH/GSSG ratio, and SOD, CAT and GPx activities were reduced in type Ⅰ endometrium cancer cells while the GSH and GSSG levels, and CAT and GPx activities were decreased and the GSH/GSSG ratio was markedly increased in type Ⅱ endometrium cancer cells. ConclusionGlutathione definitely differently affects proliferation of typeⅠand Ⅱ endometrium cancer cells, which is related to the ROS level and activities of antioxidative systems in two type of endometrium cancer cells.

Key words: Endometrium neoplasms; Reactive oxygen species; Glut athione; Antioxidative systems

CLC Number: 

  • R737.33
[1] LIANG Ting-ting， ZHANG Ping， MA De-mei， ZHU Lin. Effect on apoptosis mediated by TRAIL against Caski cells of HPV16-E5-targeted siRNA [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 24-28.
[2] FENG Wen1, FANG Yu1, YANG Cheng-xi2. Expressions of ERCC1 and C-erbB2 in endometrial carcinoma tissue and their impact on the therapeutic effect of integrated Chinese and Western medicine [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 81-84.
[3] LIU Yang1, LI Wei-hua1, L Qing-tao2, YANG Ning1, JIANG Jie1. Expression of DACH1, fatty acid synthase and sterol regulatory element binding protein-1 in endometrial carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(4): 71-76.
[4] SUN Gao-ying1, WANG Xiao-yuan2, ZHANG Xiao-jin1, HAO Xiu-yu1， GUO Yuan-fang1, WANG Xiao1, BI Wen-xiang1. Effects of N-acetylcysteine  on resistance to paclitaxel in cervical carcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 42-47.
[5] ZHANG Bing-zhen, DAI Bing-qin, ZHANG Xing-liang, WANG Zhi-ping. Associations of maternal methionine synthase A2756G and methionine synthase reductase A66G polymorphisms with neural tube defects of the offspring: A meta-analysis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(7): 125-132.
[6] ZHANG Xing-liang1, XU Li2, WANG Zhi-ping1. Risk factors of cervical cancer： A meta-analysis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 155-160.
[7] NAN Fang-fang1， WEI Shuang-yan1， JIANG Jie2， CHENG Lei3. Expression of cyclin D1 protein in endometrial carcinoma and its clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(11): 151-155.
[8] MA Ben-hong1, ZHANG Gui-yu1, LIANG Jing1, ZU Zeng-yan2, LI Yue1. Expression and significance of the HMGB1 protein  in endometrial adenocarcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 144-148.
[9] LUAN Li1, GUAN Shang-hui1, YANG Sheng-si1, CHENG Huan-huan2,4, WANG Na-na1, DONG Zhao-gang3,4, CHENG Yu-feng1. Application of WCX magnetic beads to serum proteome profiling in endometrial carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(5): 85-89.
[10] Niyaz·Hurshidam, ZHAO Hua-rong, Abdulla·Mangnisha, BAO Yong-xing. A randomized clinical trial in comparison of concurrent single agent cisplatin, cisplatin in combination with 5-fluorouracil and cisplatin in combination  with taxel for locally advanced cervical squamous cell cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(5): 128-131.
[11] YIN Hai-qin1, WEI Yu-ping2, WANG Xiao-yuan3， PENG Nan1, BI Wen-xiang2, KONG Feng2, ZHANG Pei-hai1. Comparison of antioxidant abilities of cervical squamous  carcinoma and adenocarcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 55-.
[12] YIN Hai-qin1, WEI Yu-ping2, WANG Xiao-yuan3， PENG Nan1, BI Wen-xiang2, KONG Feng2, ZHANG Pei-hai1. Comparison of antioxidant abilities of cervical squamous  carcinoma and adenocarcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 55-.
[13] ZU Zengyan1, ZHANG Guiyu1, MA Benhong1, WANG Hongxia2 . Inhibitory effect of rosiglitazone combined with cisplatin on the growth of endometrial carcinoma in Nude Mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 62-66.
[14] ZHENG Ni1, WANG Chuanxin1, DENG Xiaomei2, ZHANG Jian1, KAN Shifen1,ZHANG Xin1, LI Wei1, DU Lutao1. Expression of HLA-G in cervical cancer and its clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(2): 128-130.
[15] DING Ning, BI Wen-Xiang, KONG Feng, ZHANG Pei-Hai. Antioxidant ability of type I and type II endometrial cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(5): 63-66.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd