JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2009, Vol. 47 ›› Issue (9): 53-57.

• Articles • Previous Articles     Next Articles

Effect of silencing Notch1 on proliferation of bladder cancer cell line T24

AI Xing1, ZHANG Xiuping2, WU Zhun3, LIU Shuanglin3, ZANG Tong1, ZH ANG Guohui1, SUN Fengling1, ZHANG Shuiwen1, ZHANG Yong1, ZHANG Xu4   

  1. 1. Department of Urology, General Hospital of Beijing Military Comm and of Chin ese PLA, Beijing 100700; 
    2. Center of Medical Laboratory Diagnosis, Jinan Centra l Hospital, School of Clinical Medicine, Shandong University, 
    Jinan 250013, China;
    3. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong U niversity of Science and Technology, Wuhan 430030, China; 
    4. Department of Urology, Chinese PLA General Hospital, Beijing 100086, China
  • Received:2009-03-03 Published:2009-09-16

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Abstract:

 To investigate the effect of silencing Notch1 gene on cell proliferation in vitro in bladder cancer cell line T24. MethodsThe siRNA eukaryotic expression vector of Notch1（psiRNA1）was constructed using pGenesil plasmid. psiRNA1 was transfected into bladder cancer cell line T24 by lipofectamine. Cell growth, cycle and apoptosis of T24 were determined with methabenzthiazuron (MTT) and flow cytometry (FCM) assay after transfection. The mRNA and protein expressions of Notch1 were determined by RTPCR and western blot. ResultsCompared with the control group, the growth curve of T24 cells was inhibited after transfection, which was timedependent during 60 hours. Notch1 silencing produced a cell cycle arrest at G0/G1（P＜0.05）. The apoptotic cell index also increased to some degree. mRNA and protein expressions of Notch1 were significantly   downregulated
compared with the controls after transfection（P＜0.05）.     Conclusion  Notch1 could inhibit the proliferation of bladder cancer cells by silencing expression of Notch1

, and it might act as a carcinogenic gene in bladder cancer. 

Key words: Gene， Notch1; T24; Bladder neoplasms; Proliferation

CLC Number: 

  • R737.14
[1] NI Yong-liang, LI Qing. Clinical effectiveness of dendritic cells therapy combined with  intravesical  perfusion of epirubicin on bladder cancer after TURBT [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 114-118.
[2] GAO Li1, ZHANG Tian-yu1, TAN Ning2, ZHANG Lin-gang1. Effects of the CO2-independent cell culture system on the proliferation of  bladder cancer cells T-24 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(10): 60-64.
[3] . Relativity of TLR4 expression in uroepithelium carcinoma of the bladder [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(10): 80-82.
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