Abstract:

To investigate endogenous neurogenesis and learning and memory in hippocampul dentate gyrus of Dgalactose induced aging mice by intracerebroventricular injection of noggin protein. Method40 mature KM male mice were randomly divided into the normal control group， the aging model group， the treatment control group and the noggin treatment group. In the aging model group, treatment control group and treatment group, Dgalactose (125?mg·kg-1·d-1)  was given to mice for 6 weeks by subcutaneous injection. Noggin protein was injected into the lateral cerebral ventricle for 7 days after 6 weeks in the noggin treatment group, and saline was injected into the lateral cerebral ventricle in the treatment control group. Learning and memory abilities were measured by a Ymaze test 7 days after the operation. Neurogenesis was observed with BrdU immunohistochemical staining and the number of BrdUpositive cell were statistically analyzed in the hippocampal dentate gyrus. ResultsLearning and memory abilities in the aging control group were significantly decreased compared with the normal control group (P<0.05) but in the noggin treatment group were significantly increased compared with the aging model group and treatment control group (P<0.05). BrdUpositive neurons in the noggin treatment group were increased compared with the aging control group and treatment control group (P<0.05). ConclusionNoggin protein can improve learning and memory abilities of the aging model mice, which may be related to Dgalactose inducing impairment of neurogenesis and enhancing neurogenesis in the hippocampal dentate gyrus. 

Key words:  Galactose; Hippocampus; Neurons; Learning; Memory；  Noggin protein; Mice