Abstract:

To investigate the protective effect of curcumin on the development of acute necrotizing pancreatitis in mice. MethodsKunming mice were randomly divided into the control group, ANP group, low concentration of curcumin group, and high concentration of curcumin group. At 12h after induction of ANP, a blood sample was withdrawn for analysis of amylase activity and TNFα level, the serum amylase was determined by an Automatic Biochemical Analyzer, and expression of TNFα was determined by ELISA. Pancreatic pathological damages were evaluated to observe the pathological score. Expression of NFκB was determined by RTPCR and Western blot methods. Results  The serum amylase, TNFα level, pathological scores and expression of NFκB in the ANP group were significantly higher than the in control group（P＜0.05）,　but they were significantly lower in the two roglitazone curcumin groups than in the ANP group（P＜0.05）. ConclusionCurcumin may effectively alleviate pancreas injury in acute necrotizing pancreatitis in mice by inhibition of NFκB expression.

Key words: Pancreatitis, acute necrotizing; Cerulein; Curcumin; NFkappa B; Tumor necrosis factor; Mice