Abstract:

To explore the mediation pathway of human mesangial cell (HMC) extracellular matrix (ECM) excretion induced by ET1. MethodsMesangial cells were cultured in DMEM medium with or without ET1. Collagen type IV (Col IV) and fibronectin (Fn) in the supernatant were detected by enzymelinked immumoadsorbent assay (ELISA), expression of plateletderived growth factorB (PDGFB) mRNA was determined by reverse transcription polymerase chain response (RTPCR), and expression of the protein synthesis of PDGFB was determined by Westernblot. Results① After ET1 stimulation, compared with the control, Col IV and Fn in the  supernatant were increased, PDGFB mRNA and protein expressions were upregulated（P<0.05）, and RG50864 prevented ET1induced ECM secretion（P<0.05）, but had no effect on PDGFB expression. ② Losartan could decrease the PDGFB mRNA and protein expressions, and partly prevented ET1induced ECM secretion（P<0.05）. Conclusions ①  ET1 is able to stimulate HMC to synthesize and secrete ECM and PDGFB, the ECM secretion induced by ET1 may be partially mediated by PDGFB. ② Losartan can inhibit ET1induced ECM secretion by affecting expression of PDGFB.

Key words: Endothelin1； Extracellular matrix； Plateletderived growth factorB；