Abstract:

To establish a fluorogenic quantitative RTPCR (FQRTPCR) method for determining expressions of OATPB and OATPDmRNA, and to investigate expressions of OATPB and OATPDmRNA in lung neoplasms and nrmal lung tissues. MethodsThe OATP PCR product was subcloned into the PGEMT vector to construct recombinant plasmids. After optimizing the thermocycling conditions, a quantitative RTPCR assay was successfully developed to quantify OATP expression on the LineGene realtime PCR detection system, which was confirmed by 40 samples of lung cancer and normal lung tissues. ResultsThe standard curve was successfully established, and the linear regression value was0.998.  Using FQRTPCR, we can get an accurate quantitative result of the OATPB and OATPDmRNA expressions. Moreover, expression levels of OATPB and OATPDmRNA in lung neoplasms were higher than those in normal lung tissues. ConclusionFQRTPCR for detecting expressions of OATPB and OATPDmRNA was sucessfully established, and is accurate and sensible. 

Key words: Polymerase chain reaction； Fluorogenic quantitative； OATP gene; TA clone