Abstract:

To explore the relationship between cytochrome P450 enzyme 2E1(CYP2E1), glutathione Stransferase theta 1(GSTT1) genetic polymorphism and individual susceptibility to nonHodgkin lymphoma (NHL). MethodsCYP2E1 genotypes were detected by the polymerase chain reactionrestriction fragment length polymorphism（PCRRFLP） technique in 254 patients with NHL and 254 healthy controls. GSTT1 genotypes were detected with the polymerase chain reaction（PCR）　technique in the two groups. ResultsCYP2E1 gene polymorphisms were not statistically different between controls and patients with NHL(P>0.05). Frequencies of GSTT1 null genotypes were significantly increased in the NHL group compared with the  control group (48.0% vs 37.8%, P<0.05). In subtype analysis, individuals carrying the  GSTT1 null genotype had a 1.78fold risk of diffuse large Bcell lymphoma(DLBCL) (95% CI: 1.122.82, P=0.02) and a 1.69fold risk of follicular lymphoma (FL)（95%CI: 1.032.77, P=0.04）compared with the wild genotype. Combined genotype analysis showed that individuals carrying the CYP2E1wild genotype and GSTT1 null genotype had a 1.83fold risk of NHL(95%CI:1.14  2.95，P=0.01). ConclusionGSTT1 polymorphism may be an independent risk factor of NHL. CYP2E1 wildtype in combination with GSTT1 nulltype further increases the incidence of NHL.

Key words: NonHodgkin lymphoma; Gene polymorphism; Cytochrome P450 enzyme; Glutathione Stransferase