JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2009, Vol. 47 ›› Issue (11): 64-67.

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Specific siRNA inhibits HPV16 E7 gene expression: an experimental study

ZHANG Xiao, WANG Chuanxin, LI Wei, ZHENG Guixi, ZHANG Jian, KAN Shifeng， WANG Lishui   

  1. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2009-03-06 Published:2009-11-16

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Abstract:

To screen an effective HPV16 E7 specific siRNA and investigate its effect on expression of HPV16 E7 mRNA and cell surface HLA class I antigen in HaCaTE7 cells. MethodsThree HPV16 E7 specific siRNAs were chemically synthesized and transfected into HaCaTE7 cells by Lipofectamine2000. Expression of HPV16 E7 mRNA was determined by realtime PCR while expression of cell surface HLA class I antigen was determined by flow cytometry（FCM）.  ResultsAll three siRNAs effectively inhibited expression of HPV16 E7 mRNA in HcaCaTE7 cells. Of the siRNAs, siRNA2 was the most effective and its inhibitory rate reached 75%. Expression of cell surface HLA class I antigen was obviously upregulated. Posttransfected average mean fluorescence intensity (MFI) of HaCaTE7 cell surface HLA class I antigen was 130.18±1.07, which was significantly higher than that in the mock control group (100.32±3.01) and the nonspecific siRNA control group (100.82±2.87). ConclusionHPV16 E7 specific siRNA can effectively inhibit expression of

Key words: Papillomavirus， human； HLA antigens; Genes; RNA interference; HaCaT cells

CLC Number: 

  • R737.33
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