Abstract:

To investigate the effects of different culture media on the proliferation in vitro of  bone marrowderived mesenchymal stem cells. MethodsBone marrow was abstracted from the intertrochanter of the femur of 2month New Zealand rabbits and purification of mesenchymal stem cells was determined by a density gradient centrifugation method. The cells were cultured in 4 kinds of culture media: DMEMhigh glucose(10％FBS), DMEMlow glucose(10％FBS), DMEMlow glucose(15％FBS) and DMEMlow glucose(20％FBS). Cells of passage one were detected the expansion multiple and cells of passage two measured the growth and mineralization of cells. ResultsThe expansion multiple was 16.20±1.60 and the colonyforming number(CFN) was 6.11±1.17 in the DMEMlow glucose(15％FBS) group, which were significantly higher than those in the other three groups. ConclusionDMEMlow glucose (15%FBS) is more suitable for cultivation of MSCs than the other three media.

Key words: Bone marrowderived mesenchymal stem cells; Culture medium; Proliferation