JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• 无栏目 • Previous Articles     Next Articles

Mesenchymal stem cells combined with rapamycin immunoregulates
the spleniclymphocytes in allogenic mice

YIN Yujun1, LI Jing2, TANG Yu2, YOU Haiyan3, ZHU Wei4,　XU Wengrong4, JIAO Zhijun3
   

  1. （1. Department of Dermatology, 2. Department of Rheumatology, 3. Department Laboratory, Affiliated Hospital of Jiangsu University,
    Zhenjiang 212001, Jiangsu, China； 4. School of Medical Technology, Jiangsu University, Zhenjiang 212013, Jiangsu, China）
  • Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
  • Contact: LI Jing

PDF (PC)

119

Abstract: To investigate the immunoregulatory effects of mesenchymal stem cells(MSCs) combined with rapamycin on T and B cells in vitro and its potential mechanism. MethodsMSCs were isolated and cultivated from the bone marrow of 56 weekold BALB/c mice in vitro. The purity was detected through surface markers by flow cytometry(FCM). Splenic lymphocytes were isolated by EZSep(tm) Mouse 1X. Under ConA or LPS stimulation, lymphocytes were treated with MSCs and/or rapamycin. The proliferation was assessed by MTT coloremetry. FCM was used to analyze the apoptosis and surface markersCD69， CD28 and CD86. mRNA expressions of cytokines were detected by realtime quantitative PCR. ResultsBoth MSCs and MSCs combined with rapamycin could significantly inhibit proliferation of lymphocytes and the apoptosis of T cells. However, the inhibitory effect of the latter was more obvious than that of the former (P＜0.01). Both MSCs and MSCs combined with rapamycin could significantly increase expression of interferon (IFN)γ mRNA but decrease expression of interleukin (IL)10. No differences of CD69, CD28 and CD86 expressions were observed among all groups. ConclusionMSCs combined with rapamycin could downmodulate the function of T and B cells, and the potential mechanism may be related to its effect on the proliferation，apoptosis and mRNA expressions of IFNγ and IL10.

Key words: Mesenchymal stem cells, Rapamycin, Lymphocytes, Immunoregulatory

CLC Number: 

  • R392
[1] LI Jingyuan, SONG Ling, LIN Song, SONG Hui. Effects of carbon monoxide releasing molecule-3 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 31-37.
[2] LIU Ning, L(¨overU)Xin, LI Dong, HUNAG Zhiwei, LIU Yi, ZHANG Leling. Construction and identification of lentiviral vector for angiopoietin-1 gene and its expression in human umbilical cord mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(12): 1-7.
[3] WANG Na, CHEN Naiyao. Effect of umbilical cord mesenchymal stem cells on microglial polarization and proliferation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 16-20.
[4] FU Haiyan, HU Zhansheng, DU Hongyang, LI Chao, BAO Cuifen. Effect of rehmannia glutinosa polysaccharide on the differentiation and proliferation of over-expressed Notch1(NICD) gene in rat bone marrow mesenchymal stem cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 34-40.
[5] LIU Juan, HAN Guoqing, LIU Hui, QIN Chengyong. Mechanism of human umbilical cord mesenchymal stem cells suppressing cholangiocarcinoma cell growth [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 1-5.
[6] HU Su1,2, PANG Shu-guang2, CUI Ying2, YU Chun-xiao1, ZHAO Jia-jun1, GUAN Qing-bo1. Osteogenic potential of bone marrow mesenchymal stem cells from streptozotocininduced diabetic rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(8): 7-12.
[7] WANG Tong, LIU Yu-guang, ZHANG Liang-wen, WANG Hong-wei, LI Lian-ling, LIU Zhi-guo. Effect of inhibiting EGFR and mTOR signaling pathway on C6 glioma and its stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 37-43.
[8] CHEN Yan1, LI Dong2, ZHANG Zhe1, SHI Qing2, WANG Da-kun2, LIU Huan2, WANG Guo-yun1. Effects of human umbilical cord mesenchymal stem cells on nerve fibers in the lesions of rat endometriosis  model [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 48-51.
[9] SUN Yuan-xin1, QIN Xue-mei1, LIU Xi-min2, WANG Li-zhi2, LIU Chuan-fang1, LIU Lu1. Relationship between regulatory T cells, IL-35 and aGVHD after allo-HSCT [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 89-94.
[10] DU Hong-yang1, LI Dong-ning1， FU Hai-yan2, BAO Cui-fen2, QIN Shu-jian1. Effect of rehmannia glutinosa polysaccharide on Notch signal pathway in rat bone marrow mesenchymal stem cells during differentiation into neuron-like cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(12): 1-6.
[11] CHEN Shuo1, WANG Min1, CHEN Jian2, QING Ying1, LIN Ying-min1, LIU Liang3, QI Ying-ying4. Regulations of rapamycin on gene expressions of UDP-glucuronosyltransferase 1A isforms and cytochrome P450 3A4 induced by sulforaphane in human colon cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(11): 30-36.
[12] SHANG Xiao1, CUI Yuan-xiao1, LIU Xiao-yu1, FENG Xiao-ya2. Experimental study of human umbilical cord mesenchymal stem cells combined with minimal hematoma aspiration in the therapy of rats with intracerebral hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 10-14.
[13] LIU Hua1,2, CHEN Ya-na3, ZHENG Yan1, CHEN Li4, LI Cai-xin4, ZHUANG Xiu-ping4, WANG Yun-shan1,2. Effects of human umbilical cord mesenchymal stem cells on the growth of Hela cell lines [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(06): 29-33.
[14] LIU Ying1, LI Xiao-li1, XU Hua2, WANG Shan1, ZHANG Min1, LI Heng1, DUAN Rui-sheng1. Effects of lower dose of atorvastatin on experimental autoimmune myasthenia gravis inrats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(7): 26-31.
[15] HAN Yan-xin, CONG Ya-qin, WANG Zhi-min. Immuno-suppressive effects mediated by mesenchymal stem cells of
myelodysplastic syndrome patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 97-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd