Abstract:

To investigate the expression of Tribbles (TRBs) in human monocytedriven macrophages and to analyze the regulative role of oxidized low density lipoprotein (ox-LDL) on TRB1, TRB2 and TRB3. MethodsPrimary human monocyte-driven macrophages were cultured in vitro. Macrophages were incubated with ox-LDL and low density lipoprotein (LDL) at various concentrations for various times. The level of TRBs mRNA was determined by quantification real-time RT-PCR. ResultsAll three TRBs genes (TRB1, TRB2 and TRB3) were expressed in human monocyte-driven macrophages, among them, TRB3 was predominant, and TRB1 and TRB2 were little expressed. OxLDL increased the levels of TRB3 andTRB2 mRNA, while it decreased the expression of TRB1 in a dose-and time-dependent manner. Conclusions All three TRBs are expressed in human monocytedriven macrophages and can be regulated by ox-LDL, so TRBs, especially TRB3 may participate in the course of macrophage apoptosis.

Key words: gene, Tribbles; Macrophages; Oxidized low-density lipoprotein')">gene, Tribbles; Macrophages; Oxidized low-density lipoprotein