Abstract: To investigate the effect of a selfmade lysing solution in flow cytometry on red blood cells, and on surface markers of lymphocytes, monocytes and granulocytes. Methods： K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a selfmade lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14+， CD45+ on lymphocytes, monocytes and granulocytes were detected. Results： Both the selfmade lysing solution and the FACS Lysing solution could lyse red blood cells completely, but not destroy leukocytes. In FSC/SSC dot plot, there were three clusters of cells: lymphocytes, monocytes and granulocytes. The expression of CD14+ on these cells were (1.01±0.24)% and (1.00±0.23)%, (92.80±1.98)% and (92.81±1.94)%, (91.16±3.68)% and (91.22±3.76)% respectively； and the expression of CD45+ on these cells were (98.94±0.71)% and (98.90±0.72)%, (95.15±2.73)% and (95.11±2.76)%, (99.39±0.48)%and (99.41±0.47)% respectively. The expression of surface markers on leukocytes was not significantly different between the selfmade lysing solution and the FACS lysing solution(P＞0.05). Conclusion： The selfmade lysing solution can lyse red blood cells completely ,but not destroy leukocytes and influence the expression of surface antigens on lymphocytes, monocytes and granulocytes significantly.

Key words: Lysing solution, Flow cytometry, Antigens, CD14, Antigens, CD45