Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (7): 27-33.doi: 10.6040/j.issn.1671-7554.0.2022.1434

• 基础医学 • Previous Articles    

Effects of Dexmedetomidine on the gene expressions of SH-SY5Y cells by RNA high-throughput sequencing

GUAN Wei1,2, BAI Yunfeng1, DU Shengjie1, WANG Yuelan3, LI Ximing1,2   

  1. 1. Department of Anesthesiology, Linyi Peoples Hospital, Linyi 276000, Shandong, China;
    2. Jiangsu Province Key Laboratory of Anesthesiology and Analgesia, Xuzhou 221004, Jiangsu, China;
    3. Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong, China
  • Published:2023-07-04

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Abstract: Objective To investigate the potential mechanism of Dexmedetomidine in brain protection by analyzing its effects on the gene expression of SH-SY5Y cells. Methods SH-SY5Y cells were cultured and divided into the experimental group(T group, biological replicate n=3)and control group(C group, biological replicate n=3). The experimental group was stimulated with Dexmedetomidine for 48 hours, while the control group was treated with DMSO under the same conditions. RNA was extracted with Trizol reagent and sequenced with Illumina Novaseq. The differentially expressed genes between the two groups were identified with Limma, and analyzed with gene ontology(GO)enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis. Results A total of 211 differentially expressed genes were identified, including 105 up-regulated and 106 down-regulated ones. The expressions of FGF1, GNG13 and P2RY4 were significantly different between the two groups, and the difference folds were 2.23, 2.00 and 6.56, respectively. GO enrichment analysis showed that the differentially expressed genes were mainly enriched in phospholipase C-activating G protein-coupled receptor signaling pathway, regulation of protein kinase B signaling and BMP signaling pathway, while KEGG pathway enrichment analysis revealed that they were mainly enriched in leukocyte transendothelial migration. Conclusion Genes including FGF1, GNG13 and P2RY4 may be involved in the protective effects of Dexmedetomidine on brain. Dexmedetomidine protects the central nervous system through inflammatory mechanism.

Key words: Dexmedetomidine, RNA high-throughput sequencing, SH-SY5Y cells, Brain protection, Central nervous system

CLC Number: 

  • R614
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