Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (11): 61-66.doi: 10.6040/j.issn.1671-7554.0.2021.0222

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Correlation between the copy number of plasma free mitochondrial DNA and severity of burn patients

XU Xiaofeng, WANG Jianzhen, YANG Zehua   

  1. Laboratory of the First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China
  • Published:2021-11-11

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Abstract: Objective To investigate the changes of plasma free mitochondrial DNA(mtDNA)copy number in patients with different degrees of burns and its role in the severity of burns. Methods In accordance with the burn degree and total body surface area(TBSA), 30 patients with severe burns and 30 patients with moderate burns were selected. Another 30 healthy subjects were enrolled as controls. Plasma samples were collected on day 1, days 8-11, and days 18-21. DNA was extracted, and the copy numbers of plasma free mtDNA were detected with real-time fluorescent quantitative PCR. The correlation among mtDNA copy number, acute physiology and chronic health evaluation Ⅱ score(APACHE Ⅱ), multiple organ dysfunction syndrome score(MODS), TBSA, Ⅲ° burn area, burn index and biochemical indicators were analyzed, and Logistic regression was conducted. Results On day 1 of admission, the severe burn group had higher copy number of plasma free mtDNA than the other two groups, and the free mtDNA copy number reached the peak on days 8-11 and then decreased. The copy number of mtDNA was positively correlated with total burn size, Ⅲ° burn area, burn index, APACHE Ⅱ score and MODS(P<0.05), positively correlated with biochemical indicators such as aspartate aminotransferase, alanine aminotransferase, total bilirubin, procalcitonin, and white blood cells(P<0.01), but negatively correlated with total protein, albumin and prealbumin(P<0.01). Logistic regression analysis showed that APACHE Ⅱ score and mtDNA copy number on day 1 of admission were independent risk factors for burn severity(P<0.05). The area under the ROC curve for the diagnosis of burn degree of mtDNA copy number was 0.904, with a sensitivity of 0.833 and a specificity of 0.867. Conclusion The copy number of plasma free mtDNA in burn patients is correlated with the degree of burn and a number of biochemical indexes, which can provide references for the clinical classification of degree of burn.

Key words: Degree of burn, Free mitochondrial DNA, Real-time fluorescent quantitative PCR, Burn condition, Biochemical index

CLC Number: 

  • R644
[1] Alinejad F, Momeni M, Keyvani H, et al. Introduction to a case of orf disease in a burn wound at Motahari Hospital[J]. Ann Burns Fire Disasters, 2018, 31(3): 243-245.
[2] McKeever L, Bonini M, Braunschweig C. Feeding during phases of altered mitochondrial activity: a theory[J]. JPEN J Parenter Enteral Nutr, 2018, 42(5): 855-863.
[3] Hirata Y. Reactive oxygen species(ROS)signaling: regulatory mechanisms and pathophysiological roles[J]. Yakugaku Zasshi, 2019, 139(10): 1235-1241.
[4] Johansson PI, Nakahira K, Rogers AJ, et al. Plasma mitochondrial DNA and metabolomic alterations in severe critical illness[J]. Crit Care, 2018, 22(1): 360.
[5] 中国医师协会烧伤医师分会《烧伤感染诊治指南》编辑委. 烧伤感染的诊断标准与治疗指南(2012版)[J]. 中华烧伤杂志, 2012, 28(6): 401-403.
[6] Zheng J, Cui NH, Zhang S, et al. Leukocyte mitochondrial DNA copy number and risk of thyroid cancer: a two-stage case-control study[J]. Front Endocrinol, 2019, 10: 421. doi:10.3389/fendo.2019.00421.
[7] Qin C, Gu J, Liu R, et al. Release of mitochondrial DNA correlates with peak inflammatory cytokines in patients with acute myocardial infarction[J]. Anatol J Cardiol, 2017, 17(3): 224-228.
[8] Fujimoto C, Yamasoba T. Mitochondria-targeted antioxidants for treatment of hearing loss: a systematic review[J]. Antioxidants(Basel), 2019, 8(4): 109.
[9] Annesley SJ, Fisher PR. Mitochondria in health and disease[J]. Cells, 2019, 8(7): 680.
[10] Yang Y, Karakhanova S, Hartwig W, et al.Mitochondria and mitochondrial ROS in cancer: novel targets for anticancer therapy[J]. J Cell Physiol, 2016, 231(12): 2570-2581.
[11] Pohjoismäki JL, Goffart S. The role of mitochondria in cardiac development and protection[J]. Free Radic Biol Med, 2017, 106: 345-354. doi:10.1016/j.freeradbiomed.2017.02.032.
[12] Akbari M, Kirkwood TBL, Bohr VA. Mitochondria in the signaling pathways that control longevity and health span[J]. Ageing Res Rev, 2019, 54: 100940. doi:10.1016/j.arr.2019.100940.
[13] 李凤娇, 顾雯, 俞捷, 等. 线粒体自噬机制、相关疾病及中药对其调节作用的研究进展[J]. 中国药房, 2018, 29(20): 2865-2871.
[14] Gao L, Liu X, Zhang D, et al. Early diagnosis of bacterial infection in patients with septicopyemia by laboratory analysis of PCT, CRP and IL-6[J]. Exp Ther Med, 2017, 13(6): 3479-3483.
[15] Zuo KJ, Medina A, Tredget EE. Important developments in burn care[J]. Plast Reconstr Surg, 2017, 139(1): 120-138.
[16] Yuan Y, Chen Y, Peng T, et al. Mitochondrial ROS-induced lysosomal dysfunction impairs autophagic flux and contributes to M1 macrophage polarization in a diabetic condition[J]. Clin Sci(Lond), 2019, 133(15): 1759-1777.
[17] Pandi P, Jain A, Raju S, et al. Therapeutic approaches for the delivery of TNF-α siRNA[J]. Ther Deliv, 2017, 8(5): 343-355.
[18] 王鹿, 李丽萍, 邢欣, 等. 肝脏巨噬细胞及其在肝损伤和修复中的作用[J]. 中国临床药理学与治疗学, 2018, 23(10): 114-121.
[19] 雷玮, 王丹丹. 肝损伤血清标志物研究进展[J]. 实用肝脏病杂志, 2017, 20(2): 252-256.
[20] Reznichenko A, Korstanje R. The role of platelet-activating factor in mesangial pathophysiology[J]. Am J Pathol, 2015, 185(4): 888-896.
[21] Dan Dunn J, Alvarez LA, Zhang X, et al. Reactive oxygen species and mitochondria: a Nexus of cellular homeostasis[J]. Redox Biol, 2015, 6: 472-485. doi:10.1016/j.redox.2015.09.005.
[22] Kan C, Ungelenk L, Lupp A, et al. Ischemia-reperfusion injury in aged livers-the energy metabolism, inflammatory response, and autophagy[J]. Transplantation, 2018, 102(3): 368-377.
[23] 任华建, 胡琼源, 任建安. 线粒体DNA损伤在缺血再灌注中的研究进展[J]. 中华实验外科杂志, 2019, 36(4): 786-789.
[24] Chen YH, Wang ZG, Yu MM, et al. The predictive ability of liver function indexes on 18F-FDG uptake in the liver[J]. Nuklearmedizin Nucl Med, 2017, 56(6): 239-242.
[25] Liang FF, Wang J, Li L, et al. Chronic liver disease increases with damage to intestinal barrier function[J]. Zhonghua Gan Zang Bing Za Zhi, 2018, 26(8): 612-617.
[26] 赖晓敏, 熊爱兵. 严重烧伤后中性粒细胞、巨噬细胞功能的变化及其影响[J]. 中外医疗, 2014, 33(11): 195.
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