Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (11): 61-66.doi: 10.6040/j.issn.1671-7554.0.2021.0222

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Correlation between the copy number of plasma free mitochondrial DNA and severity of burn patients

XU Xiaofeng, WANG Jianzhen, YANG Zehua   

  1. Laboratory of the First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China
  • Published:2021-11-11

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Abstract: Objective To investigate the changes of plasma free mitochondrial DNA(mtDNA)copy number in patients with different degrees of burns and its role in the severity of burns. Methods In accordance with the burn degree and total body surface area(TBSA), 30 patients with severe burns and 30 patients with moderate burns were selected. Another 30 healthy subjects were enrolled as controls. Plasma samples were collected on day 1, days 8-11, and days 18-21. DNA was extracted, and the copy numbers of plasma free mtDNA were detected with real-time fluorescent quantitative PCR. The correlation among mtDNA copy number, acute physiology and chronic health evaluation Ⅱ score(APACHE Ⅱ), multiple organ dysfunction syndrome score(MODS), TBSA, Ⅲ° burn area, burn index and biochemical indicators were analyzed, and Logistic regression was conducted. Results On day 1 of admission, the severe burn group had higher copy number of plasma free mtDNA than the other two groups, and the free mtDNA copy number reached the peak on days 8-11 and then decreased. The copy number of mtDNA was positively correlated with total burn size, Ⅲ° burn area, burn index, APACHE Ⅱ score and MODS(P<0.05), positively correlated with biochemical indicators such as aspartate aminotransferase, alanine aminotransferase, total bilirubin, procalcitonin, and white blood cells(P<0.01), but negatively correlated with total protein, albumin and prealbumin(P<0.01). Logistic regression analysis showed that APACHE Ⅱ score and mtDNA copy number on day 1 of admission were independent risk factors for burn severity(P<0.05). The area under the ROC curve for the diagnosis of burn degree of mtDNA copy number was 0.904, with a sensitivity of 0.833 and a specificity of 0.867. Conclusion The copy number of plasma free mtDNA in burn patients is correlated with the degree of burn and a number of biochemical indexes, which can provide references for the clinical classification of degree of burn.

Key words: Degree of burn, Free mitochondrial DNA, Real-time fluorescent quantitative PCR, Burn condition, Biochemical index

CLC Number: 

  • R644
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