Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (2): 72-78.doi: 10.6040/j.issn.1671-7554.0.2019.1154

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Detection of tumor cells, carbohydrate antigen 153, carbohydrate antigen 125 and carcinoembryonic antigen in peripheral blood circulation in 105 cases of ductal carcinoma in situ or with minimal invasive carcinoma

GUAN Yanfei1, CHEN Zhiqiang2, YUAN Chunlei1, PENG Jianming1, MI Xianjun2, LIU Jun3, CHEN Ang2, YUAN Bin1, LIU Zicheng1   

  1. 1. Department of Clinical Laboratory, Zhongshan BOAI Hospital Affiliated to Southern Medical University, Zhongshan 528400, Guangdong, China;
    2. Department of Pathology, Zhongshan BOAI Hospital Affiliated to Southern Medical University, Zhongshan 528400, Guangdong, China;
    3. Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou 510060, Guangdong, China
  • Online:2020-02-10 Published:2022-09-27

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Abstract: Objective To explore the diagnostic value of peripheral blood circulation tumor cell(CTC), carbohydrate antigen 153(CA153), carbohydrate antigen 125(CA125), carcinoembryonic antigen(CEA)on single or combined detection of ductal carcinoma in situ(DCIS)or with microinvasive carcinoma. Methods A total of 136 patients with benign breast disease(benign group), 105 patients with DCIS or with micro invasive cancer(malignant group)and 151 healthy people(control group)were selected as the study objects. The amount of CTC was measured by immunofluorescence in situ hybridization(IMFISH). The values of CA153, CA125 and CEA were measured by electrochemiluminescence. The value of single detection(CA153, CA125, CEA and CA153+CA125+CEA)and combined detection(CTC+CA153+CA125+CEA)in diagnosing DCIS or micro invasive cancer was analyzed. Results (1)For all the detection methods, the positive rate of malignant group was significantly higher than those in benign group and control group(P<0.01). (2)Compared with the single detection methods, the positive rate of combined detection method in malignant group was significantly higher(all P<0.001). (3) Compared with the control group and benign group, the detection values of CTC, CA153 and CA125 methods in malignant group were higher(P<0.001). (4) The sensitivity, specificity and coincidence rate were significantly different among the combined detection method and the single detection methods(P<0.001). (5) The areas under the curve of CTC, CA153, CA125, CEA,(CA153+CA125+CEA)and(CTC+CA153+CA125+CEA)detections were 0.873, 0.803, 0.775, 0.760, 0.900 and 0.959, respectively. Conclusion CTC+CA153+CA125+CEA detection method has a higher sensitivity and coincidence rate in the diagnosis of DCIS or with micro invasive cancer, larger diagnostic area of ROC curve and a better specificity.

Key words: Circulating tumor cell, Carbohydrate antigen 153, Carbohydrate antigen 125, Carcinoembryonic antigen, Ductal carcinoma in situ, Minimally invasive carcinoma, Tumor markers

CLC Number: 

  • R446.1
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