JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (11): 22-26.doi: 10.6040/j.issn.1671-7554.0.2017.099

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Intermittent tensile stress affects the expression of TRPV5 in murine osteoclasts

PENG Fei1, SONG Ling2, QI Xiangmin3, LI Yaqin2, DIAO Yanfei2, ZHAO Shoufeng4, WANG Lili4, YANG Fang2, LU Shulai2, LÜ Wenfang2, GUO Dawei2   

  1. 1. Weifang Medical University, Weifang 261000, Shandong, China;
    2. Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266071, Shandong, China;
    3. School of Stomatology, Shandong University, Jinan 250012, Shandong, China;
    4. Central Laboratory, Qingdao Municipal Hospital, Qingdao 266071, Shandong, China
  • Received:2017-01-24 Online:2017-11-10 Published:2017-11-10

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Abstract: Objective To investigate the expression of transient receptor potential vanilloid receptor V(TRPV5)during the differentiation of osteoclasts mediated by intermittent tensile stress. Methods Murine osteoclasts were divided into 4 groups and mediated by different loading stress: group A(1 000 με), group B(3 000 με), group C(5 000 με), and group D(0 με). The mRNA expression of TRPV5 in the 4 groups was detected with fluorescence quantitative PCR before and after the differentiation. Results Compared with group D, the other 3 groups had higher mRNA expression of TRPV5(P<0.001). Pairwise comparison results showed that there was no statistical difference between group B and C(P>0.05),there was statistical difference between any other two groups(P<0.05). Conclusion Intermittent tensile stress promotes the expression of TRPV5 channel gene in osteoclasts within a certain range.

Key words: Intermittent tensile stress, Osteoclast, Transient receptor potential vanilloid receptor 5, Fluorescence quantitative PCR

CLC Number: 

  • R782
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