JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (5): 23-30.doi: 10.6040/j.issn.1671-7554.0.2016.919

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Layer-by-layer assembly delivered VEGF enhances biocompatibility of decellularized scaffolds

TAN Qi1, LI Na2, TAO Yunming3, TANG Hao3, WU Zhongshi3   

  1. 1. Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    2. Department of Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    3. Department of Cardiac Surgery, Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, China
  • Received:2016-07-28 Online:2017-05-10 Published:2017-05-10

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Abstract: Objective Heparin/chitosan layer-by-layer(LBL)assembly delivered vascular endothelial growth factor(VEGF)on decellularized scaffolds(DS)was designed for better biocompatibility. Methods LBL was crosslinked by 1-ethyl-(3- dimethylamino propyl)carbonyl diimine(EDC), and VEGF was delivered on decellularized bovine jugular vein to produce different scaffolds. The microstructure surface of different scaffolds was observed with scanning electron microscopy(SEM). The release performance of heparin and VEGF was determined with elution test. The blood compatibility was tested with anticoagulant activity and platelet adhesion test. The endothelialization on scaffolds was analyzed with cell proliferation. Results Heparin/chitosan multilayer films formed the surface coating by nanoparticles. EDC improved the stability of multilayer films. The release rate of heparin and VEGF was lower in LBL/EDC- 山 东 大 学 学 报 (医 学 版)55卷5期 -谭琦,等.载血管内皮生长因子层层自组装提高异种去细胞血管生物相容性 \=-DS group than in LBL-DS group, and the anticoagulant activity and platelet adhesion were better in LBL/EDC-DS group than in LBL-DS group. Live cell staining and MTT test showed that LBL/EDC delivered VEGF significantly increased proliferation of endothelial cells on scaffolds. Conclusion Heparin/chitosan layer-by-layer technology delivered VEGF can improve biocompatibility and accelerate endothelialization on decellularized scaffolds.

Key words: Layer-by-layer assembly, Decellularized scaffolds, Chemical crosslink, Vascular endothelia growth factor, Biocompatibility

CLC Number: 

  • R339
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