Abstract: Objective  To introduce a method for preparation of thyroid frozen sections to improve the section quality. Methods  After sampling, the specimen was frozen and cut into sections, which were fixed immediately by the methanol-acetic acid fixative and stained by hematoxylin and eosin. Results  The staining of sections was bright-coloured, and structures were clear and complete under microscope. The contrast of blue and red staining in nucleus and cytoplasm was obvious. The cells had no obvious swelling and ice crystal formation. The rate of good-quality section was more than 98%. Conclusion  Ideal temperature of cryostat, frozen time, choice of fixative, slicing technique and staining are the keys to prepare the frozen sections with high qulities.

Key words: Temperature, Frozen section, Thyroid tissue, Fixation