关键词: 人类基因组项目 , 单核苷酸, 基因, 多态性, HLA抗原

Abstract: Objective: To analyze the polymorphism of HLA-A，-B, and -DRB1 in northern Hans of China and obtain more comprehensive and accurate data about HLA molecular genetics. Methods: The methods of polymerase chain reactionsequence specific primers and sequence specific oligonucleotide probing(PCR-SSP and -SSOP) were used to determine the HLA-A，-B, and -DRB1 alleles in 8924 unrelated healthy Han individuals in northern China. Results：Low resolution alleles 18 HLA-A, 44HLA-B and 13 HLA-DRB1 including some rare HLA alleles that had not been reported in China before were discovered in this study. The most prevalent HLA types were A^*02， B^*13 and DRB1^*15, with a gene frequency of 0.2886, 0.1430 and 0.1759　respectively. The most common A-B haplotype in this study was A^*30B^*13 with a haplotype frequency of 0.0895. The most common HLA-A-B-DRB1 haplotype was A^*30B^*13DRB1^*07 with a haplotype frequency of 0.0742. Conclusion: HLA-A, -B， and -DRB1 alleles in the northern Hans of China are high polymorphic. Large scale DNA based on HLA genotyping contributes to the detection of low frequency alleles.

Key words: HLA antigens, Genes, Polymorphism, single nucleatide, Human genome projectHLA