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山东大学学报 (医学版) ›› 2020, Vol. 58 ›› Issue (3): 81-86.doi: 10.6040/j.issn.1671-7554.0.2019.1257

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CD151-MUT突变对肺腺癌细胞A549迁移的影响及机制

杨秀婷1,刘启功2,左萍2,刘正湘2,左后娟2   

  1. 1.滨州医学院附属医院心血管内科 山东 滨州 256600;2. 华中科技大学同济医学院附属同济医院心血管内科, 湖北 武汉 430030
  • 出版日期:2020-03-10 发布日期:2022-09-27
  • 通讯作者: 左后娟. E-mail:zuohoujuan@126.com
  • 基金资助:
    国家自然科学基金(81873535)

Effect of CD151-MUT mutation on migration of lung adenocarcinoma cell line A549 and its mechanism

YANG Xiuting1, LIU Qigong2, ZUO Ping2, LIU Zhengxiang2, ZUO Houjuan2   

  1. 1. Department of Cardiology of Binzhou Medical University Hospital, Binzhou 256600, Shandong, China;
    2. Department of Cardiology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
  • Online:2020-03-10 Published:2022-09-27

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摘要： 目的 研究CD151整合素结合缺陷突变体(CD151-MUT)突变对肺腺癌细胞A549迁移的影响及机制。 方法 分别运用CD151、CD151-MUT和GFP质粒转染肺腺癌A549细胞,作为CD151组、CD151-MUT组和GFP组,同时设置Control组及Reagent组。改良的Boyden趋化小室法检测各组细胞的体外迁移能力。Western blotting方法检测各组细胞CD151蛋白、黏着斑激酶(FAK)及P130Cas蛋白的表达。 结果 (1) CD151组(2.23±0.22)和CD151-MUT组(2.15±0.17)CD151蛋白的表达较Control组(1.03±0.15)、GFP组(1.18±0.13)和Reagent组(1.22±0.11)均增高,5组均值差异有统计学意义(F=39.8,P<0.001),但两两多重比较显示CD151组和CD151-MUT组间差异无统计学意义(q=1.007, P=0.628)。(2)CD151组较Control组、GFP组及Reagent组促进肿瘤细胞的迁移(F=15.3, P=0.007),而CD151-MUT组细胞的迁移能力较CD151组下降,差异有统计学意义(q=6.760,P=0.010)。(3)CD151高表达促进p-FAK、p-P130Cas蛋白表达增加(F=16.3,P<0.001; F=10.9,P=0.001),而CD151-MUT组p-FAK 、p-P130Cas蛋白表达水平较CD151组减弱(q=8.620,P=0.010; q=6.859,P=0.009)。 结论 CD151-MUT突变破坏CD151-整合素复合体形成,抑制肿瘤转移。CD151-α3/α6整合素复合体在肿瘤细胞迁移过程中发挥重要作用。

关键词: 整合素, CD151, 肿瘤转移, FAK-P130Cas, 肺腺癌细胞A549

Abstract: Objective To study the effect of CD151-MUT on migration of lung adenocarcinoma cell line A549 and the mechanism involved. Methods pAAV-CD151, pAAV-CD151-MUT and pAAV-GFP were constructed and transfected into A549, and named as CD151 group, CD151-MUT group and GFP group. Meanwhile, Control group and Reagent group were set up. The migration of A549 was detected by modified Boyden chamber assay. Protein expression levels of CD151,p-focal adhesion kinase(FAK)and p-P130Cas were measured by Western blotting. Results (1)Compared with the Control group(1.03±0.15), GFP group(1.18±0.13)and Reagent group(1.22±0.11), the expression of CD151 protein was increased both in the CD151 group(2.23±0.22)and CD151-MUT group(2.15±0.17)with statistical difference(F=39.8,P<0.001), but there was no significant difference of the CD151 protein expression between CDC151 and CDC151-CDC151-MUT groups(q=1.007,P=0.628). (2) The CD151 group significantly promoted cells migration compared with the Control group, GFP group and Reagent group(F=15.3, P=0.007). However, compared with the CD151 group, the migration and invasion of A549 cells were decreased in CD151-MUT group(q=6.760,P=0.010). (3) Overexpression of the CD151 protein could promote the expressions of p-FAK and p-P130Cas proteins(F=16.3,P<0.001; F=10.9,P=0.001), while the protein expressions were reduced in the CD151-MUT group compared with CD151 group(q=8.620,P=0.010; q=6.859,P=0.009). Conclusion CD151-MUT mutation impairs the formation of CD151-integrin and inhibits the tumor metastasis. The CD151-α3/α6 integrins complex is functionally important.

Key words: Integrin, CD151, Tumor migration, FAK-P130Cas, Lung adenocarcinoma cell line A549

中图分类号: 

  • R734.2
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