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山东大学学报 (医学版) ›› 2019, Vol. 57 ›› Issue (12): 74-79.doi: 10.6040/j.issn.1671-7554.0.2019.526

• • 上一篇    

尿液α1-抗胰蛋白酶、尿转铁蛋白水平与小儿原发性肾病综合征激素治疗效果的关联分析

潘艳艳1,孙永超2,刘雪梅1,张洪霞1,周蔚然1,赵翠芬3   

  1. 1.山东大学齐鲁儿童医院儿科, 山东 济南 250022;2.济南护理职业学院护理系, 山东 济南 250203;〓3.山东大学齐鲁医院儿科, 山东 济南 250012
  • 发布日期:2022-09-27
  • 通讯作者: 赵翠芬. E-mail:zhaocuifen@sdu.edu.cn
  • 基金资助:
    山东省医药卫生科技发展计划(2015WS0443)

Correlation between urinary α1-antitrypsin and transferrin levels and hormone therapy effect on childrens primary nephrotic syndrome

PAN Yanyan1, SUN Yongchao2, LIU Xuemei1, ZHANG Hongxia1, ZHOU Weiran1, ZHAO Cuifen3   

  1. 1. Department of Pediatrics, Qilu Childrens Hospital of Shandong University, Jinan 250022, Shandong, China;
    2. Nursing Faculty, Jinan Vocational College of Nursing, Jinan 250203, Shandong, China;
    3. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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摘要： 目的 探讨尿液α1-抗胰蛋白酶(AAT)、尿转铁蛋白(TRF)水平预测小儿原发性肾病综合征(PNS)激素治疗临床效果的价值。 方法 选取山东大学齐鲁儿童医院2016年5月至2017年6月PNS住院患儿90例,根据糖皮质激素治疗4周后的反应分为激素敏感组(n=60)和激素耐药组(n=30),选取同期30 例健康儿童为正常对照组,收集20 mL新鲜晨起尿,4 ℃离心,收集上清液。收集所有患儿基本资料以及激素治疗前后的血胆固醇、血肌酐、白蛋白、24 h尿蛋白定量等生化指标。采用酶联免疫吸附试验(ELISA)法检测尿液中AAT和TRF的浓度,并经尿肌酐(Cr)校正后分析其预测PNS患儿对激素治疗效果的反应。 结果 激素敏感组和激素耐药组的尿液AAT/Cr比值和TRF/Cr比值均高于正常对照组,差异具有统计学意义(P<0.05);激素敏感组和激素耐药组的尿液AAT/Cr比值和TRF/Cr比值差异有统计学意义(P<0.05);回归分析显示,尿液AAT/Cr比值和TRF/Cr比值是影响PNS激素治疗临床效果的危险因素(P<0.05)。 结论 激素耐药PNS患儿尿液中的AAT和TRF浓度均较激素敏感患儿升高;如果PNS患儿发病初期尿液中AAT和TRF浓度升高,提示可能为激素耐药。

关键词: 尿液α1-抗胰蛋白酶, 尿转铁蛋白, 小儿原发性肾病综合征, 激素耐药, 临床疗效

Abstract: Objective To investigate the value of urinary α1-antitrypsin(AAT)and transferrin(TRF)levels in predicting the clinical efficacy of hormone therapy on children with primary nephrotic syndrome(PNS). Methods A total of 90 PNS children hospitalized in Qilu Childrens Hospital of Shandong University from May 2016 to June 2017 were studied. According to the response of glucocorticoid treatment for 4 weeks, they were divided into hormone-sensitive group (n=60)and steroid-resistant group(n=30). At the same time, 30 healthy children were selected as normal control group. Fresh morning urine(20 mL)was collected and centrifugated at 4 ℃, then supernatant was acquired. The basic information and biochemical index levels of theserum cholesterol, serum creatinine, albumin and 24 h urinary protein measured before and after hormone therapy were collected. Enzyme linked immunosorbent assay(ELISA)was used to determine the concentrations of AAT and TRF levels in urine, and the correction value by urinary creatinine(Cr) 山 东 大 学 学 报 (医 学 版)57卷12期 -潘艳艳,等.尿液α1-抗胰蛋白酶、尿转铁蛋白水平与小儿原发性肾病综合征激素治疗效果的关联分析 \=-was used to predict the response of PNS children to hormone therapy. Results Compared with normal control group, the ratios of urinary AAT/Cr and TRF/Cr in steroid-sensitive group and steroid-resistant group were higher(P<0.05). The urinary AAT/Cr ratio and TRF/Cr ratio were statistically different between steroid-sensitive group and steroid-resistant group(P<0.05). The regression analysis showed that urinary AAT/Cr ratio and TRF/Cr ratio were risk factors for the clinical efficacy of steroid therapy on PNS children(P<0.05). Conclusion The concentrations of urinary AAT and TRF in steroid-resistant children after treatment are higher than those in steroid-sensitive children. If the urinary AAT and TRF levels increase in early stage of PNS, the children may be steroid- resistant.

Key words: Urine α1-antitrypsin, Urinary transferrin, Childrens primary nephrotic syndrome, Hormone resistance, Clinical efficacy

中图分类号: 

  • R543.2
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