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山东大学学报(医学版) ›› 2017, Vol. 55 ›› Issue (8): 30-34.doi: 10.6040/j.issn.1671-7554.0.2016.413

• 基础医学 • 上一篇    下一篇

放疗后肿瘤99Tcm-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性

胡玉敬1,吴大勇1,张文艳1,边艳珠1,魏强1,田丛娜1,常胜利2   

  1. 1.河北省人民医院核医学科, 河北 石家庄 050051;2.石家庄市正定县人民医院外科, 河北 石家庄 050800
  • 收稿日期:2016-04-13 出版日期:2017-08-10 发布日期:2017-08-10
  • 通讯作者: 边艳珠. E-mail:yanzhubian99@163.com E-mail:yanzhubian99@163.com
  • 基金资助:
    河北省医学科学研究重点课题(20110206)

Correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α after radiotherapy

HU Yujing1, WU Dayong1, ZHANG Wenyan1, BIAN Yanzhu1, WEI Qiang1, TIAN Congna1, CHANG Shengli2   

  1. 1. Department of Nuclear Medicine, Hebei General Hospital, Shijiazhuang 050051, Hebei, China;
    2. Department of Surgery, Zhengding Peoples Hospital, Shijiazhuang 050800, Hebei, China
  • Received:2016-04-13 Online:2017-08-10 Published:2017-08-10

摘要: 目的 探讨2-(2-甲基-5-硝基-1H-咪唑-1-基)磷酸乙酯(99Tcm-MNLS)乏氧显像监测肿瘤乏氧状态变化与肿瘤组织乏氧诱导因子-1α(HIF-1α)表达相关性。 方法 将荷瘤小鼠按显像时间分为放疗后显像组(R12h、R24h、R36h)及对照组(C12h、C24h、C36h)。放疗组肿瘤部位均给予单次15 Gy的照射剂量,对照组不予放疗。按照各组相应时间进行99Tcm-MNLS乏氧显像,并计算肿瘤部位与对侧正常组织放射性比值(T/NT),肿瘤组织进行HIF-1α免疫组化。将T/NT比值、HIF-1α免疫组化结果进行相关性分析。 结果 免疫组化结果显示,R12h组T/NT比值、HIF-1α高于C12h组,R36h组低于C36h组,(P均<0.000),R24h组、C24h组两组比较差异无统计学意义(P=0.612、0.731)。放疗组各组T/NT比值、HIF-1α表达呈正相关(r=0.793, P=0.003),对照组各组T/NT比值、HIF-1α表达也呈正相关(r=0.756, P=0.004)。 结论 99Tcm-MNLS乏氧显像T/NT比值与HIF-1α表达水平呈正相关, 99Tcm-MNLS乏氧显像可用于肿瘤放疗后疗效评估,并可为临床确定肿瘤分割照射时间提供依据。

关键词: 放射性核素显像, 乏氧诱导因子-1α, 动物模型, 乏氧

Abstract: Objective To explore the correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α(HIF-1α)expression in the hypoxic changes of tumors after radiotherapy. Methods The tumor-bearing mice were divided into radiotherapy groups(R12h, R24h, R36h)and control groups(C12h, C24h, C36h)according to the time of developing. The radiotherapy groups received an exposure dose of 15 Gy each time, while the control groups received no radiotherapy. After 99Tcm-MNLS hypoxic scintigraphy, the radioactivity ratio of tumor to non-tumor(T/NT)was calculated. The level of HIF-1α in tumors was evaluated. After that, the correlation between T/NT and HIF-1α level was analyzed. Results The T/NT and HIF-1α level in R12h group and R36h group were higher than those in the C12h group and C36h group(all P=0.000). There was no difference between the R24h and C24h groups(P=0.612, 0.731). The T/NT was positively correlated with HIF-1α level in the radiotherapy groups(r=0.793, P=0.003), as well as in the control groups(r=0.756, P=0.004). Conclusion Uptake of 99Tcm-MNLS and expression of HIF-1α are strongly 山 东 大 学 学 报 (医 学 版)55卷8期 -胡玉敬,等.放疗后肿瘤99Tcm-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性 \=-correlated to the changes of hypoxia in tumors after radiotherapy, and 99Tcm-MNLS hypoxic scintigraphy can provide the basis for the best time of reirradiation.

Key words: Hypoxia-inducible factor 1α, Radionuclide imaging, Hypoxia, Animal models

中图分类号: 

  • R445.5
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