放疗后肿瘤99Tcm-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性

doi:10.6040/j.issn.1671-7554.0.2016.413

摘要/Abstract

摘要： 目的探讨2-(2-甲基-5-硝基-1H-咪唑-1-基)磷酸乙酯(⁹⁹Tc^m-MNLS)乏氧显像监测肿瘤乏氧状态变化与肿瘤组织乏氧诱导因子-1α(HIF-1α)表达相关性。方法将荷瘤小鼠按显像时间分为放疗后显像组(R_12h、R_24h、R_36h)及对照组(C_12h、C_24h、C_36h)。放疗组肿瘤部位均给予单次15 Gy的照射剂量,对照组不予放疗。按照各组相应时间进行⁹⁹Tc^m-MNLS乏氧显像,并计算肿瘤部位与对侧正常组织放射性比值(T/NT),肿瘤组织进行HIF-1α免疫组化。将T/NT比值、HIF-1α免疫组化结果进行相关性分析。结果免疫组化结果显示,R_12h组T/NT比值、HIF-1α高于C_12h组,R_36h组低于C_36h组,(P均<0.000),R_24h组、C_24h组两组比较差异无统计学意义(P=0.612、0.731)。放疗组各组T/NT比值、HIF-1α表达呈正相关(r=0.793, P=0.003),对照组各组T/NT比值、HIF-1α表达也呈正相关(r=0.756, P=0.004)。结论 ⁹⁹Tc^m-MNLS乏氧显像T/NT比值与HIF-1α表达水平呈正相关, ⁹⁹Tc^m-MNLS乏氧显像可用于肿瘤放疗后疗效评估,并可为临床确定肿瘤分割照射时间提供依据。

关键词: 放射性核素显像, 乏氧诱导因子-1α, 动物模型, 乏氧

Abstract: Objective To explore the correlation between ⁹⁹Tc^m-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α(HIF-1α)expression in the hypoxic changes of tumors after radiotherapy. Methods The tumor-bearing mice were divided into radiotherapy groups(R_12h, R_24h, R_36h)and control groups(C_12h, C_24h, C_36h)according to the time of developing. The radiotherapy groups received an exposure dose of 15 Gy each time, while the control groups received no radiotherapy. After ⁹⁹Tc^m-MNLS hypoxic scintigraphy, the radioactivity ratio of tumor to non-tumor(T/NT)was calculated. The level of HIF-1α in tumors was evaluated. After that, the correlation between T/NT and HIF-1α level was analyzed. Results The T/NT and HIF-1α level in R_12h group and R_36h group were higher than those in the C_12h group and C_36h group(all P=0.000). There was no difference between the R_24h and C₂₄h groups(P=0.612, 0.731). The T/NT was positively correlated with HIF-1α level in the radiotherapy groups(r=0.793, P=0.003), as well as in the control groups(r=0.756, P=0.004). Conclusion Uptake of ⁹⁹Tc^m-MNLS and expression of HIF-1α are strongly 山东大学学报 (医学版)55卷8期 -胡玉敬,等.放疗后肿瘤⁹⁹Tc^m-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性 \=-correlated to the changes of hypoxia in tumors after radiotherapy, and ⁹⁹Tc^m-MNLS hypoxic scintigraphy can provide the basis for the best time of reirradiation.

Key words: Hypoxia-inducible factor 1α, Radionuclide imaging, Hypoxia, Animal models

中图分类号:

R445.5

胡玉敬,吴大勇,张文艳,边艳珠,魏强,田丛娜,常胜利. 放疗后肿瘤⁹⁹Tc^m-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性[J]. 山东大学学报（医学版）, 2017, 55(8): 30-34.

HU Yujing, WU Dayong, ZHANG Wenyan, BIAN Yanzhu, WEI Qiang, TIAN Congna, CHANG Shengli. Correlation between ⁹⁹Tc^m-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α after radiotherapy[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 30-34.

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放疗后肿瘤⁹⁹Tc^m-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性

Correlation between ⁹⁹Tc^m-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α after radiotherapy

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