人牙根尖乳头细胞抑制人单核细胞THP-1活化及其分子机制

doi:10.6040/j.issn.1671-7554.0.2016.761

山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (3): 49-53.doi: 10.6040/j.issn.1671-7554.0.2016.761

人牙根尖乳头细胞抑制人单核细胞THP-1活化及其分子机制

闫松鹤¹,高立芬²,赵伟¹,王燕^1,3

1.山东大学口腔医学院山东省口腔组织再生重点实验室;2.山东大学基础医学院医学免疫学系;3.山东大学口腔医院特诊科, 山东济南 250012

收稿日期:2016-06-30 出版日期:2017-03-10 发布日期:2017-03-10
通讯作者: 王燕. E-mail:wangyan1965@sdu.edu.cn E-mail:wangyan1965@sdu.edu.cn
基金资助:
国家自然科学基金面上项目(81172862);山东省科技发展计划(2014GGH218030)

Inhibition of human apical papilla cells on the activation of mononuclear cell THP-1 and its molecular mechanism

YAN Songhe¹, GAO Lifen², ZHAO Wei¹, WANG Yan^1,3

1. School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration;
2. Department of Immunology, School of Basic Medical Sciences, Shandong University;
3. VIP Center, Stomatological Hospital, Shandong University, Jinan 250012, Shandong, China

Received:2016-06-30 Online:2017-03-10 Published:2017-03-10

摘要/Abstract

摘要： 目的体外研究人牙根尖乳头细胞(HAPCs)对人单核细胞系THP-1活化的影响及其分子机制。方法采用Transwell系统共培养HAPCs和THP-1,Real-time PCR检测THP-1中TNF-α、IL-12、IL-10及TGF-β1的mRNA表达量,流式细胞术检测共培养3 d THP-1中TNF-α的表达,通过Western blotting检测Stat3及Smad3磷酸化情况。结果 Real-time PCR结果显示,HAPCs抑制THP-1中TNF-α、IL-12的mRNA表达而促进IL-10、TGF-β1的mRNA表达(P<0.05);流式细胞术结果显示,共培养3 d的THP-1中TNF-α表达显著降低(P<0.05);Western blotting结果显示,共培养后HAPCs激活THP-1中的Stat3通路(P<0.05),而对Smad3通路无影响(P>0.05)。结论 HAPCs可能通过Stat3通路抑制THP-1活化。

关键词: 炎症因子, THP-1细胞, 人牙根尖乳头细胞

Abstract: Objective To investigate the effects of human apical papilla cells(HAPCs)on the mononuclear cell line THP-1 activation and the molecular mechanism in vitro. Methods HAPCs and THP-1 were co-cultured in Transwell system, and then the mRNA expressions of TNF-α, IL-12, IL-10 and TGF-β1 in THP-1 were detected with Real-time PCR. The expression of TNF-α in THP-1 3d after co-culture was determined with flow cytometry. The phosphorylation of Stat3 and Smad3 were tested with Western blotting. Results HAPCs inhibited the mRNA expressions of TNF-α and IL-12 in THP-1 and promoted the mRNA expressions of IL-10 and TGF-β1(P<0.05). The expression of TNF-α significantly decreased in THP-1 3 d after co-culture(P<0.05). HAPCs activated the Stat3 pathway in THP-1 after co-cultivation(P<0.05), but had no effect on the Smad3 pathway(P>0.05). Conclusion HAPCs may inhibit the activation of THP-1 through the Stat3 pathway.

Key words: Human apical papilla cells, Inflammatory factors, THP-1 cell

中图分类号:

R781

闫松鹤,高立芬,赵伟,王燕. 人牙根尖乳头细胞抑制人单核细胞THP-1活化及其分子机制[J]. 山东大学学报（医学版）, 2017, 55(3): 49-53.

YAN Songhe, GAO Lifen, ZHAO Wei, WANG Yan. Inhibition of human apical papilla cells on the activation of mononuclear cell THP-1 and its molecular mechanism[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 49-53.

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人牙根尖乳头细胞抑制人单核细胞THP-1活化及其分子机制

Inhibition of human apical papilla cells on the activation of mononuclear cell THP-1 and its molecular mechanism

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