雌二醇对Th1细胞极化与肝纤维化形成的影响

doi:10.6040/j.issn.1671-7554.0.2016.676

摘要/Abstract

摘要： 目的探讨雌二醇(E₂)对Th1细胞极化以及与肝纤维化发生的影响。方法根据E₂水平将47例肝纤维化患者分为A组(E₂增高组)19例和B组(E₂正常组)28例,阴性对照作为C组(正常人)30例。应用流式细胞术检测T细胞亚群,化学发光法检测透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原氨基末端肽(PⅢP)和Ⅳ型胶原(CⅣ);ELISA法检测干扰素-γ(IFN-γ)、白介素-2(IL-2)、白介素-4(IL-4)和白介素-10(IL-10),比较A、B组之间以及与C组的差异。结果 A组E₂高于C组和B组(P<0.001)。A组HA、LN、PⅢP、CⅣ均高于C组(P<0.001, P=0.001, P=0.006, P<0.001),B组HA、LN、PⅢP、CⅣ也高于C组(均为P<0.001);A组PⅢP高于B组(P=0.045)。A组CD3⁺T细胞低于C组和B组(P<0.001, P=0.001),CD4⁺T细胞也低于C组和B组(P<0.001, P=0.024),B组CD3⁺、CD4⁺T细胞低于C组(P=0.002, P<0.001);A组、B组CD8⁺T细胞高于C组(P=0.001, P=0.012)。A组IFN-γ高于C组和B组(均为P<0.001),IL-2也高于C组和B组(P<0.001, P=0.003),B组高于C组(P=0.001, P=0.012);A组IL-4低于C组和B组(P=0.002, P=0.016),IL-10也低于C组和B组(P<0.001, P=0.012)。结论高E₂在延缓肝纤维化形成的同时也通过Th1细胞极化影响着机体的免疫功能,加速了肝纤维化的发展,E₂在肝纤维化的形成过程中扮演了双重角色。

关键词: 雌二醇, Th1细胞, CD4⁺T细胞, CD8⁺T细胞, 肝纤维化

Abstract: Objective To explore the effects of estradiol(E₂)on Th1 cell polarization and liver fibrosis. Methods A total of 47 patients with liver fibrosis were divided into 2 groups according to the level of E₂: group A(elevated E₂ level, n=19)and group B(normal E₂ level, n=28). Another 30 healthy people were enrolled as the negative controls(group C). The T lymphocyte subsets were measured with flow cytometry. The levels of hyaluronic acid(HA), laminin(LN), procollagen type Ⅲ amino-terminal peptide(PⅢP)and type IV collagen(CⅣ)were detected with chemiluminescence. The levels of IFN-γ, IL-2, IL-4 and IL-10 were measured with ELISIA assay. Then the parameters of the three groups were compared. Results The E₂ level was higher in group A than in group C and group B(P<0.001). The levels of HA, LN, PIIIP and CⅣ in group A were all higher than those in group C(P<0.001, P=0.001, P=0.006, P<0.001), and the levels of these four parameters in group B were also higher than those in group C(all P<0.001). The level of PⅢP was higher in group A than in group B(P=0.045). The percentage of CD3⁺ T cells in group A was lower than that in group C and group B respectively(P<0.001, P=0.001), and the percentage of CD4⁺ T cells was also lower than that in group C and group B(P<0.001, P=0.024), while the percentages of CD3⁺ T cells and CD4⁺ T cells in group B were all lower than those in group C(P=0.002, P<0.001); The percentage of CD8⁺ T cells in both group A and groupB was higher than that in group C(P=0.001, P=0.012). The level of IFN-γ in group A was higher than that in group C and group B(both P<0.001), and the level of IL-2 was also higher than that in group C and group B(P<0.001, P=0.003), at the same time, the levels of these two 山东大学学报 (医学版)54卷8期 -展凤霞,等.雌二醇对Th1细胞极化与肝纤维化形成的影响 \=-parameters in group B were all higher than those in group C(P=0.001, P=0.012). The level of IL-4 in group A was lower than that in group C and group B(P=0.002, P=0.016), and the level of IL-10 was also lower than that in group C and group B(P<0.001, P=0.012). Conclusion E₂ played a dual role in the formation of liver fibrosis. Elevated level of E₂ accelerated liver fibrosis, and affected the immune function via Th1 cell polarization.

Key words: Estradiol, Th1 cell, CD4⁺T cell, CD8⁺T cell, Liver fibrosis

中图分类号:

R575.2

展凤霞,丁娟,马蓓蕾,王谦. 雌二醇对Th1细胞极化与肝纤维化形成的影响[J]. 山东大学学报（医学版）, 2016, 54(8): 64-68.

ZHAN Fengxia, DING Juan, MA Beilei, WANG Qian. Effects of estradiol on Th1 cell polarization and liver fibrosis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 64-68.

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