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山东大学学报(医学版) ›› 2016, Vol. 54 ›› Issue (8): 64-68.doi: 10.6040/j.issn.1671-7554.0.2016.676

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雌二醇对Th1细胞极化与肝纤维化形成的影响

展凤霞1,丁娟2,马蓓蕾2,王谦2   

  1. 1.山东大学校医院检验科, 山东 济南 250100;2.山东大学齐鲁医院检验科, 山东 济南 250012
  • 收稿日期:2016-06-06 出版日期:2016-08-10 发布日期:2016-08-10
  • 通讯作者: 王谦. E-mail:sd.wangqian@163.com E-mail:sd.wangqian@163.com
  • 基金资助:
    国家“863”计划(2014AA022304);山东省科技攻关项目(2013GSF11824)

Effects of estradiol on Th1 cell polarization and liver fibrosis

ZHAN Fengxia1, DING Juan2, MA Beilei2, WANG Qian2   

  1. 1. Department of Clinical Laboratory, Campus Clinic of Shandong University, Jinan 250100, Shandong, China;
    2. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-06-06 Online:2016-08-10 Published:2016-08-10

摘要: 目的 探讨雌二醇(E2)对Th1细胞极化以及与肝纤维化发生的影响。 方法 根据E2水平将47例肝纤维化患者分为A组(E2增高组)19例和B组(E2正常组)28例,阴性对照作为C组(正常人)30例。应用流式细胞术检测T细胞亚群,化学发光法检测透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原氨基末端肽(PⅢP)和Ⅳ型胶原(CⅣ);ELISA法检测干扰素-γ(IFN-γ)、白介素-2(IL-2)、白介素-4(IL-4)和白介素-10(IL-10),比较A、B组之间以及与C组的差异。 结果 A组E2高于C组和B组(P<0.001)。A组HA、LN、PⅢP、CⅣ均高于C组(P<0.001, P=0.001, P=0.006, P<0.001),B组HA、LN、PⅢP、CⅣ也高于C组(均为P<0.001);A组PⅢP高于B组(P=0.045)。A组CD3+T细胞低于C组和B组(P<0.001, P=0.001),CD4+T细胞也低于C组和B组(P<0.001, P=0.024),B组CD3+、CD4+T细胞低于C组(P=0.002, P<0.001);A组、B组CD8+T细胞高于C组(P=0.001, P=0.012)。A组IFN-γ高于C组和B组(均为P<0.001),IL-2也高于C组和B组(P<0.001, P=0.003),B组高于C组(P=0.001, P=0.012);A组IL-4低于C组和B组(P=0.002, P=0.016),IL-10也低于C组和B组(P<0.001, P=0.012)。 结论 高E2在延缓肝纤维化形成的同时也通过Th1细胞极化影响着机体的免疫功能,加速了肝纤维化的发展,E2在肝纤维化的形成过程中扮演了双重角色。

关键词: 雌二醇, Th1细胞, CD4+T细胞, CD8+T细胞, 肝纤维化

Abstract: Objective To explore the effects of estradiol(E2)on Th1 cell polarization and liver fibrosis. Methods A total of 47 patients with liver fibrosis were divided into 2 groups according to the level of E2: group A(elevated E2 level, n=19)and group B(normal E2 level, n=28). Another 30 healthy people were enrolled as the negative controls(group C). The T lymphocyte subsets were measured with flow cytometry. The levels of hyaluronic acid(HA), laminin(LN), procollagen type Ⅲ amino-terminal peptide(PⅢP)and type IV collagen(CⅣ)were detected with chemiluminescence. The levels of IFN-γ, IL-2, IL-4 and IL-10 were measured with ELISIA assay. Then the parameters of the three groups were compared. Results The E2 level was higher in group A than in group C and group B(P<0.001). The levels of HA, LN, PIIIP and CⅣ in group A were all higher than those in group C(P<0.001, P=0.001, P=0.006, P<0.001), and the levels of these four parameters in group B were also higher than those in group C(all P<0.001). The level of PⅢP was higher in group A than in group B(P=0.045). The percentage of CD3+ T cells in group A was lower than that in group C and group B respectively(P<0.001, P=0.001), and the percentage of CD4+ T cells was also lower than that in group C and group B(P<0.001, P=0.024), while the percentages of CD3+ T cells and CD4+ T cells in group B were all lower than those in group C(P=0.002, P<0.001); The percentage of CD8+ T cells in both group A and groupB was higher than that in group C(P=0.001, P=0.012). The level of IFN-γ in group A was higher than that in group C and group B(both P<0.001), and the level of IL-2 was also higher than that in group C and group B(P<0.001, P=0.003), at the same time, the levels of these two 山 东 大 学 学 报 (医 学 版)54卷8期 -展凤霞,等.雌二醇对Th1细胞极化与肝纤维化形成的影响 \=-parameters in group B were all higher than those in group C(P=0.001, P=0.012). The level of IL-4 in group A was lower than that in group C and group B(P=0.002, P=0.016), and the level of IL-10 was also lower than that in group C and group B(P<0.001, P=0.012). Conclusion E2 played a dual role in the formation of liver fibrosis. Elevated level of E2 accelerated liver fibrosis, and affected the immune function via Th1 cell polarization.

Key words: Estradiol, Th1 cell, CD4+T cell, CD8+T cell, Liver fibrosis

中图分类号: 

  • R575.2
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