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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (10): 16-20.doi: 10.6040/j.issn.1671-7554.0.2014.1000

• 基础医学 • 上一篇    下一篇

Caveolin-1对脑缺血大鼠血管新生的影响

郑娟1, 李政2, 张文静1, 袁琳冉1, 樊书菠1, 刘玉刚1, 袁中瑞1   

  1. 1. 山东大学医学院病理生理学教研室, 山东 济南 250012;
    2. 昆明医科大学第二附属医院, 云南 昆明 650101
  • 收稿日期:2014-12-26 出版日期:2015-10-10 发布日期:2015-10-10
  • 通讯作者: 刘玉刚。E-mail:liu.yugang@sdu.edu.cn;袁中瑞。E-mail:zhongruiyuan@sdu.edu.cn E-mail:liu.yugang@sdu.edu.cn;zhongruiyuan@sdu.edu.cn
  • 基金资助:
    国家自然科学基金(81171106,81201391);中国博士后科学基金(20110491578)

Role of Caveolin-1 in postischemic angiogenesis in a rat model of ischemic stroke

ZHENG Juan1, LI Zheng2, ZHANG Wenjing1, YUAN Linran1, FAN Shubo1, LIU Yugang1, YUAN Zhongrui1   

  1. 1. Department of Pathophysiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. The Second Hospital of Kunming Medical University, Kunming 650101, Yunnan, China
  • Received:2014-12-26 Online:2015-10-10 Published:2015-10-10

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摘要： 目的 探讨缺血性脑卒中大鼠缺血周边脑组织Caveolin-1(Cav-1)的表达及抑制其表达对缺血周边血管新生的影响。方法 随机将雄性Wistar大鼠分为4组:假手术组(Sham组,n=15),脑缺血组(MCAO组,n=55),Cav-1 siRNA慢病毒转染组(siRNA组,n=20)以及非编码siRNA慢病毒转染组(misRNA组,n=20)。用Q-PCR以及免疫印迹检测Cav-1的动态表达,FITC-dextran检测血管密度,多普勒血流仪检测血流量。结果 与Sham组比较,MCAO组再灌注4 h~3 d, Cav-1表达逐渐下降(P均<0.05),3 d表达水平最低(P<0.01),7 d 后明显恢复(P<0.05),14 d接近正常(P>0.05)。与MCAO组比较,siRNA组Cav-1的表达水平显著降低,并且缺血周边血管密度及血流量显著下降(P均<0.05);misRNA组的上述指标无明显变化。结论 Cav-1参与调节缺血性脑卒中后缺血周边脑组织的血管新生。

关键词: Caveolin-1, 血管新生, 大鼠, 缺血性脑卒中

Abstract: Objective To investigate the expression and involvement of Caveolin-1(Cav-1) in postischemic angiogenesis using a rat model of ischemic stroke. Methods Normal male Wistar rats were randomly divided into four groups: Sham group(n=15), cerebral ischemia group (MCAO group, n=55), lentivirus-expressing Cav-1 siRNA treated group (siRNA group, n=20) and lentivirus-expressing nonsense siRNA treated group (misRNA group, n=20). Q-PCR and Western blotting analysis were used to detect the expression of Cav-1. FITC-dextran was used to detect the vessel volume, and Laser Doppler was used to measure the local cerebral blood flow in ischemic penumbral. Results The expressions of Cav-1 from hour 4 to day 3 post-MCAO significantly decreased in MCAO group (all P<0.05), compared with sham group, and the minimum level appeared on day 3 post-MCAO(P<0.01). Interestingly, the expression of Cav-1 on day 7 post-MCAO significantly restored (P<0.05), and reached the normal level on day 14 post-MCAO(P>0.05). As expected, in Cav-1 siRNA group, the expression of Cav-1 significantly decreased compared with MCAO or misRNA group (all P<0.05). Meanwhile, there were significantly weakened vessel volume and local cerebral blood flow in ischemic penumbral (P<0.05). Conclusion Cav-1 might be involved in postischemic angiogenesis. Further investigation of the regulation molecular machenism of Cav-1 in postischemic angiogenesis may be helpful for therapeutic angiogenesis for ischemic stroke.

Key words: Rats, Angiogenesis, Ischemic stroke, Caveolin-1

中图分类号: 

  • R614.2
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