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山东大学学报(医学版) ›› 2014, Vol. 52 ›› Issue (1): 100-103.doi: 10.6040/j.issn.1671-7554.0.2013.462

• 临床医学 • 上一篇    下一篇

考试压力对大学生唾液中CCL28与sIgA的影响

戴美璐,肖孟,赵兵杰,张蒙蒙,姜广水   

  1. 山东大学口腔医学院综合科 山东省口腔生物医学重点实验室,济南 250012
  • 收稿日期:2013-07-25 出版日期:2014-01-10 发布日期:2014-01-10
  • 通讯作者: 姜广水,E-mail:gjiang@sina.cn
  • 基金资助:

    2011年度国家级大学生创新创业训练计划项目(1110422067)。

Effect of academic stress on CCL28 and sIgA in saliva of undergraduates1

DAI Mei-Lu, XIAO Meng, ZHAO Bing-Jie, ZHANG Meng-Meng, JIANG Guang-Shui   

  1. Department of Endodontics and Periodontics, Stomatology Hospital of Shandong University, School of Stomatology,
    Shandong University; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China
  • Received:2013-07-25 Online:2014-01-10 Published:2014-01-10

摘要:

目的  通过检测大学生考试前后唾液中黏膜相关上皮趋化因子( mucosa-associated epithelia chemokine, CCL28/MEC)与分泌型免疫球蛋白A (secretory immunoglobin A, sIgA)含量的变化,探讨考试应激对口腔黏膜免疫功能的影响。方法  收集37名大学生(17名大二女生,10名大三男生和10名大三女生,后两者分别设为男生组和女生组)考试前后的非刺激性全唾液, 采用酶联免疫吸附试验检测唾液中CCL28与sIgA水平的变化。结果  考后37名学生唾液中CCL28与sIgA的吸光度值低于考前水平,差异有统计学意义(P<0.05);女生组比男生组的考试前后唾液CCL28水平的变化更显著(P<0.05);女生组考试前后唾液中CCL28水平的变化比sIgA更为明显(P<0.05),而男生组考试前后唾液中两指标间变化的差异不明显。结论   考试应激能促进唾液中sIgA与CCL28水平的升高,女性比男性更容易受考试应激的影响。

关键词: 考试应激, 分泌型免疫球蛋白 A, 唾液, 黏膜相关上皮趋化因子

Abstract:

 Objective  To investigate the effect of academic stress on oral mucosal immune function by semi-quantitatively detecting and comparing the levels of mucosa-associated epithelia chemokine (CCL28/MEC) and secretory immunoglobin A (sIgA) in saliva before and after certain exams. Methods  A total of 37 undergraduates (17 sophomore girls, 10 junior boys and 10 junior girls, the latter two were defined as the male group and the female group ) were enrolled to collect non-stimulated completed saliva before and after certain exams. The changes of CCL28 and sIgA in the samples were detected with ELISA. Results  The OD values of sIgA and CCL28 after exams significantly decreased (P<0.05) in all 37 students. The change of CCL28 (P<0.05) in the saliva spanning the exams was more significant in the female group than in the male group, whereas the change of sIgA level (P<0.05) showed no obvious difference. After the same exam, the change of CCL28 in saliva was more obvious than the change of sIgA in the female group (P<0.05), without significant difference in the male group (P>0.05). Conclusion  Academic stress plays an important role in the secretion of CCL28 and sIgA in saliva, and females are more susceptible to such stress.

Key words: Secretory immunoglobin A, Mucosa-associated epithelia chemokine, Saliva, Academic stress

[1] 江虹,张茜,孙钰,潘芳 . 医科新生状态-特质焦虑与人格、应对方式及唾液皮质醇的相关性[J]. 山东大学学报(医学版), 2008, 46(9): 866-868.
[2] 李 潇,郑 燕,李 冬,姜广水 . 人唾液CCL28与sIgA抗体分泌的相关性研究[J]. 山东大学学报(医学版), 2007, 45(1): 65-67.
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