关键词: 幽门螺杆菌；生物膜；二烯丙基三硫醚；抗菌活性

Abstract:

ObjectiveTo investigate the antimicrobial effects of diallyl trisulfide (DATS) on Helicobacter pylori(H.pylori) biofilms and to explore the drug-resistance mechanism of biofilms. Methods  The minimal inhibitory concentration of selected antimicrobials were measured by broth microdilution assay; The biofilm models in vitro were established on agar plate; The cell activity of planktonic and sessile H.pylori treated with selected antimicrobials was measured with XTT reduction assay; The morphology and activity changes of H.pylori in deep biofilm were observed by confocal laser scanning microscopy. Results  Both clarithromycin and DATS elicited strong antimicrobial activity against planktonic and sessile H.pylori; Different concentrations of clarithromycin significantly reduced the cell activity of H.pylori biofilm; The cell activity of the DATS treated group was significantly lower than that of the control group and the clarithromycin treated group. It had statistical differences. Conclusion  DATS elicits strong antimicrobial activity against both planktonic and sessile H.pylori. It may act as an alternative chemotherapeutic agent for multidrug-resistant bacterial strains.

Key words: H.pylori; Biofilm; Diallyl trisulfide; Antimicrobial activity