关键词: 盐霉素；结肠癌干细胞；增殖；耐药；克隆球

Abstract:

Objective   To investigate the impacts of Salinomycin on colon cancer stem cell (cCSC). Methods    The cCSC was screened by culturing the clone spheres in Serum Free Medium (SFM). Further, we detected the targeting of Salinomycin to cCSC and studied the impacts of Salinomycin on the characteristics of cCSC including proliferation ability, drug resistence, clone sphere forming ability, expression level of stem cell proteins. Results   We acquired cCSC successfully by sphere culturing in SFM and established the ideal cCSC model. The cCSC showed triple sensitivity to Salinomycin than HCT116+/+ cell. After treatment by Salinomycin, the double time of cCSC increased from (1.21±0.15) days to (1.54±0.04) days; the IC50 to 5-FU declined from (40.21±3.02) μmol/L to (20.28±2.15) μmol/L, and the IC50 to oxaliplatin declined from (14.23±1.08) μmol/L to (7.64±0.53) μmol/L; the clone sphere forming rate declined from (0.42±0.01)% to (0.08±0.01)%, and the expression level of ABCG2, SOX2 and OCT4 also declined significantly. Conclusion    Salinomycin possesses significant targeting to cCSC, and can inhibit the proliferation ability, drug resistence, clone sphere forming ability, expression level of stem cell proteins of cCSC. This provides new theory basis for cCSC therapy.

Key words: Salinomycin; Colon cancer stem cell; Proliferation; Drug resistence; Clone sphere