AIF和PARP-1在庆大霉素致前庭毛细胞凋亡中的作用

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (8): 49-52.

AIF和PARP-1在庆大霉素致前庭毛细胞凋亡中的作用

张道宫1，樊兆民1，韩月臣1，刘闻闻2,3，白晓卉2,3，李亚伟1，徐磊1，王明明1，罗建芬1，李建峰2,3，王海波1,2,3

1.山东大学附属省立医院耳鼻喉科，济南 250021； 2.山东省临床医学研究院眼耳鼻喉科学研究所，济南 250021；
3.山东省耳科学重点实验室，济南 250021

收稿日期:2013-01-10 出版日期:2013-08-10 发布日期:2013-08-10
通讯作者: 王海波， E-mail：whbotologic797@163.com
基金资助:
国家自然科学基金（81200744）；山东省科技发展计划项目（2011GGH21839）

Apoptosis-inducing factor and PARP-1 involving in gentamicin-induced vestibular hair cell death

ZHANG Dao-gong1, FAN Zhao-min1, HAN Yue-chen1, LIU Wen-wen2,3, BAI Xiao-hui2,3, LI Ya-wei1, XU Lei1, WANG Ming-ming1, LUO Jian-fen1, LI Jian-feng2,3, WANG Hai-bo1,2,3

1. Department of Otorhinolaryngology & Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University,
Jinan 250021, China; 2． Institute of Eye and ENT, Shandong Clinic Research Institute, Jinan 250021, China;
3. Shandong Provincial Key Laboratory of Otology, Jinan 250021, China

Received:2013-01-10 Online:2013-08-10 Published:2013-08-10

摘要/Abstract

摘要：

目的探讨凋亡诱导因子（AIF）和聚(ADP-核糖)聚合酶1（PARP-1）在庆大霉素损伤致前庭毛细胞凋亡中的作用及机制。方法取出生后3~4d的大鼠球囊斑和椭圆囊斑行离体前庭器官培养，经培养过夜后再用2mg/mL庆大霉素培养液继续培养72h。用流式细胞术检测前庭毛细胞凋亡，RT-PCR法检测AIF和PARP-1基因表达情况， Western blotting法分别检测AIF线粒体蛋白和胞浆蛋白的表达，Western blotting法检测PARP-1蛋白表达。结果流式细胞术结果显示庆大霉素组前庭毛细胞凋亡率较对照组显著增加。RT-PCR显示庆大霉素损伤组AIF和PARP-1表达明显增加。Western blotting结果显示庆大霉素损伤组线粒体蛋白表达降低，而胞浆蛋白表达升高。庆大霉素损伤组PARP-1蛋白表达增加。结论 AIF介导的非Caspase依赖途径参与了庆大霉素损伤导致前庭毛细胞凋亡，PARP-1可能参与了AIF通路的激活。

关键词: 凋亡诱导因子；前庭毛细胞；凋亡

Abstract:

Objective To investigate the possible involvement of AIF and PARP-1 in gentamicin-induced vestibular hair cell death. Methods Vestibular organ obtained from postnatal day (p) p3 or p4 rats were maintained in tissue culture medium and were exposed to 2 mg/mL gentamicin (GM) for up to 72 h. Apoptosis was determined by flow cytometry (FCW). Expressions of AIF and PARP-1 mRNA were determined by RT-PCR. Expressions of the mitochondrial protein and cytoplasm protein of AIF were detected by Western blotting. The expression of PARP-1 protein was detected by Western blotting. Results GM induced apoptosis in vestibular hair cells. The number of apoptotic cells increased as shown by FCW. RT-PCR showed an up-regulation of AIF and PARP-1 mRNA in the GM group. Western blotting results showed that mitochondrial protein of AIF was decreased after gentamicin exposure while cytoplasm protein of AIF was increased. Expression of protein of PARP-1 was increased after gentamicin exposure. Conclusion AIF participates in gentamicin-induced apoptosis of vestibular hair cells. PARP-1 might participate in the action of AIF pathway.

Key words: Apoptosisinducing factor； Vestibular sensory cell； Apoptosis

中图分类号:

R764.5

张道宫1，樊兆民1，韩月臣1，刘闻闻2,3，白晓卉2,3，李亚伟1，徐磊1，王明明1，罗建芬1，李建峰2,3，王海波1,2,3. AIF和PARP-1在庆大霉素致前庭毛细胞凋亡中的作用[J]. 山东大学学报(医学版), 2013, 51(8): 49-52.

ZHANG Dao-gong1, FAN Zhao-min1, HAN Yue-chen1, LIU Wen-wen2,3, BAI Xiao-hui2,3, LI Ya-wei1, XU Lei1, WANG Ming-ming1, LUO Jian-fen1. Apoptosis-inducing factor and PARP-1 involving in gentamicin-induced vestibular hair cell death[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(8): 49-52.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed