您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (06): 34-39.

• 基础医学 • 上一篇    下一篇

抑癌基因PDLIM4在前列腺癌细胞中的差异表达

李先哲1,司曼飞1,2,郭艳霞1,苑辉卿1   

  1. 山东大学医学院 1.生物化学与分子生物学研究所, 济南 250012;
    2.临床医学2009级五年制, 济南 250012
  • 收稿日期:2013-03-27 出版日期:2013-06-10 发布日期:2013-06-10
  • 通讯作者: 苑辉卿, Email:lyuanhq@sdu.edu.cn

Differential expression and regulation of PDLIM4 in prostate cancer cells

LI Xian-zhe1, SI Man-fei1,2, GUO Yan-xia1, YUAN Hui-qing1   

  1. 1. Department of Biochemistry and Molecular Biology; 
    2. Clinical medicine 2009, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2013-03-27 Online:2013-06-10 Published:2013-06-10

摘要:

目的   探讨PDLIM4基因在正常前列腺上皮细胞(RWPE1)、前列腺癌细胞(PC-3、 DU145、LNCaP)、前列腺癌多药耐药细胞(PC-3/Doc)及其他耐药细胞(K562/A02、H460/Doc)中的差异表达。方法   采用定量PCR(qPCR)、Western blot、免疫荧光等方法检测PDLIM4基因在各种细胞株中的表达;利用Western blo和DNA甲基转移酶(DNMTs)活性检测分析细胞中DNMTs的表达及活性。结果   PDLIM4基因在RWPE1高表达,在DU145、LNCaP细胞中基本无表达,在前列腺癌PC-3细胞中表达甚低,而在与其对应的多药耐药PC-3/Doc细胞中高表达(P<0.05),并且在耐药细胞株中的高表达具有组织特异性。DNMTs在RWPE1表达水平低于前列腺癌细胞,但在PC-3和PC-3/Doc中无差异。酶活性分析表明,PC-3/Doc中DNMTs的活性低于PC-3细胞(P<0.05)。结论   PDLIM4基因在前列腺癌细胞中低表达,这种特异性的在前列腺癌耐药细胞中表达显著上调可能是由DNMTs活性降低所致。

关键词: 基因,PDLIM4;5-氮杂-2’-脱氧胞苷;多西紫杉醇;前列腺肿瘤

Abstract:

Objective   To analyze the differential expression of tumor suppressor PDLIM4 gene in nonneoplastic RWPE1 cells and prostate cancer (PC) cell lines and different drug-resistant cell lines.  Methods   Different cell lines including RWPE1, carcinoma cell lines (PC-3, DU145, and LNCaP), multidrug-resistant cells (PC-3/Doc, K562/A02, H460/Doc) were used to identify PDLIM4 expression by quantitative PCR (qPCR), Western blotting, and immunofluorescence (IF) assays.  The expressions and activities of DNA methyltransferases (DNMTs) were also tested in PC-3 and PC-3/Doc cells by Western blotting and enzyme activity assays. Results   PDLIM4 was overexpressed in RWPE1 cells, not expressed in DU145 or LNCaP cells, slightly detected in PC-3 cells but predominantly evidenced in its multidrug resistant PC-3/Doc cells, and this kind of high expression of PDLIM4 in drug-resistant cell lines is tissuespecific. Enzyme activity assays demonstrated that the activity of DNMTs were significantly reduced in PC-3/Doc compared to PC-3 cells, even though the expression levels of DNMTs were similar in both cell lines.  Conclusion   The expressions of PDLIM4 were dramatically reduced in PC cell lines, while this tissue-specific up-regulation of PDLIM4 in PC-3/Doc might result from the decreased enzyme activity of DNMTs when compared to PC-3 cells.

Key words: Gene,  PDLIM4; 5-Aza-2’-deoxycytidine; Docetaxel; Prostate cancer

中图分类号: 

  • R349.6
[1] 蒋慧慧1,胡志敏2,李先哲1,席广民1,蒋汉明1,苑辉卿1. 人PDLIM4基因启动子报告基因载体的构建及其在人类肿瘤细胞中的表达[J]. 山东大学学报(医学版), 2011, 49(5): 19-.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!