抑癌基因PDLIM4在前列腺癌细胞中的差异表达

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (06): 34-39.

抑癌基因PDLIM4在前列腺癌细胞中的差异表达

李先哲1，司曼飞1,2，郭艳霞1，苑辉卿1

山东大学医学院 1.生物化学与分子生物学研究所，济南 250012；
2.临床医学2009级五年制，济南 250012

收稿日期:2013-03-27 出版日期:2013-06-10 发布日期:2013-06-10
通讯作者: 苑辉卿， Email:lyuanhq@sdu.edu.cn

Differential expression and regulation of PDLIM4 in prostate cancer cells

LI Xian-zhe1, SI Man-fei1,2, GUO Yan-xia1, YUAN Hui-qing1

1. Department of Biochemistry and Molecular Biology;
2. Clinical medicine 2009, School of Medicine, Shandong University, Jinan 250012, China

Received:2013-03-27 Online:2013-06-10 Published:2013-06-10

摘要/Abstract

摘要：

目的探讨PDLIM4基因在正常前列腺上皮细胞(RWPE1)、前列腺癌细胞(PC-3、 DU145、LNCaP)、前列腺癌多药耐药细胞(PC-3/Doc)及其他耐药细胞(K562/A02、H460/Doc)中的差异表达。方法采用定量PCR(qPCR)、Western blot、免疫荧光等方法检测PDLIM4基因在各种细胞株中的表达；利用Western blo和DNA甲基转移酶(DNMTs)活性检测分析细胞中DNMTs的表达及活性。结果 PDLIM4基因在RWPE1高表达，在DU145、LNCaP细胞中基本无表达，在前列腺癌PC-3细胞中表达甚低，而在与其对应的多药耐药PC-3/Doc细胞中高表达(P<0.05)，并且在耐药细胞株中的高表达具有组织特异性。DNMTs在RWPE1表达水平低于前列腺癌细胞，但在PC-3和PC-3/Doc中无差异。酶活性分析表明，PC-3/Doc中DNMTs的活性低于PC-3细胞(P<0.05)。结论 PDLIM4基因在前列腺癌细胞中低表达，这种特异性的在前列腺癌耐药细胞中表达显著上调可能是由DNMTs活性降低所致。

关键词: 基因，PDLIM4；5-氮杂-2’-脱氧胞苷；多西紫杉醇；前列腺肿瘤

Abstract:

Objective To analyze the differential expression of tumor suppressor PDLIM4 gene in nonneoplastic RWPE1 cells and prostate cancer (PC) cell lines and different drug-resistant cell lines. Methods Different cell lines including RWPE1, carcinoma cell lines (PC-3, DU145, and LNCaP), multidrug-resistant cells (PC-3/Doc, K562/A02, H460/Doc) were used to identify PDLIM4 expression by quantitative PCR (qPCR), Western blotting, and immunofluorescence (IF) assays. The expressions and activities of DNA methyltransferases (DNMTs) were also tested in PC-3 and PC-3/Doc cells by Western blotting and enzyme activity assays. Results PDLIM4 was overexpressed in RWPE1 cells, not expressed in DU145 or LNCaP cells, slightly detected in PC-3 cells but predominantly evidenced in its multidrug resistant PC-3/Doc cells, and this kind of high expression of PDLIM4 in drug-resistant cell lines is tissuespecific. Enzyme activity assays demonstrated that the activity of DNMTs were significantly reduced in PC-3/Doc compared to PC-3 cells, even though the expression levels of DNMTs were similar in both cell lines. Conclusion The expressions of PDLIM4 were dramatically reduced in PC cell lines, while this tissue-specific up-regulation of PDLIM4 in PC-3/Doc might result from the decreased enzyme activity of DNMTs when compared to PC-3 cells.

Key words: Gene， PDLIM4; 5-Aza-2’-deoxycytidine; Docetaxel; Prostate cancer

中图分类号:

R349.6

李先哲1，司曼飞1,2，郭艳霞1，苑辉卿1. 抑癌基因PDLIM4在前列腺癌细胞中的差异表达[J]. 山东大学学报(医学版), 2013, 51(06): 34-39.

LI Xian-zhe1, SI Man-fei1,2, GUO Yan-xia1, YUAN Hui-qing1 . Differential expression and regulation of PDLIM4 in prostate cancer cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(06): 34-39.

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