儿童B系急性淋巴细胞白血病树突状细胞的生物学特性

doi:10.6040/j.issn.1671-7554.0.2014.303

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (11): 60-64.doi: 10.6040/j.issn.1671-7554.0.2014.303

儿童B系急性淋巴细胞白血病树突状细胞的生物学特性

庄泳¹, 李栋², 付金秋¹, 时庆², 鞠秀丽¹

1. 山东大学齐鲁医院儿童医疗中心山东济南 250012;
2. 山东大学齐鲁医院低温医学研究室, 山东济南 250012

收稿日期:2014-05-09 发布日期:2014-11-10
通讯作者: 鞠秀丽.E-mail:shellysdcn07@gmail.com E-mail:shellysdcn07@gmail.com
基金资助:
山东省自然科学基金(ZR2011HM007);山东省科技攻关计划(2013GSF11812)

Biological features of dendritic cells derived from childhood B lineage acute lymphoblastic leukemia in vitro

ZHUANG Yong¹, LI Dong², FU Jinqiu¹, SHI Qing², JU Xiuli¹

1. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
2. Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China

Received:2014-05-09 Published:2014-11-10

摘要/Abstract

摘要： 目的体外诱导培养正常儿童与B系急性淋巴细胞白血病(B-ALL)儿童的树突状细胞(DC),比较二者的生物学特性.方法分离10例B-ALL初诊患儿(ALL组)和10例正常儿童(对照组)的外周血单个核细胞,以rhGM-CSF(20 ng/mL)、rhIL-4(10 ng/mL)及TNF-α(10 ng/mL)联合培养8 d,显微镜下观察细胞形态,流式细胞仪检测细胞免疫表型(CD11c、CD80、CD83、CD86),ELISA检测培养上清中IL-12的浓度,混合淋巴细胞反应(MLR)检测抗原递呈功能,电化学法检测上清中葡萄糖浓度.结果 ALL组细胞未表现出DC的典型形态,CD11c、CD80、CD83、CD86表达均较对照组细胞低(P< 0.05),分泌IL-12的能力弱于对照组细胞(P<0.05),刺激T淋巴细胞增殖的能力弱于对照组细胞,ALL组细胞培养上清中的葡萄糖浓度高于对照组(P<0.05).结论 B-ALL来源的DC成熟度异常,功能减弱,葡萄糖代谢异常可能是其成熟异常的原因之一.

关键词: 葡萄糖代谢, 白血病,急性, 淋巴系, 树突状细胞

Abstract: Objective To induce and culture dendritic cells (DC) derived from normal children and childhood B lineage acute lymphoblastic leukemia (B-ALL) patients, and compare their biological features in vitro. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from 10 B-ALL patients (ALL group) and 10 healthy donors (control group). The isolated PBMCs were co-cultured with rhGM-CSF(20 ng/mL), rh IL-4(10 ng/mL) and TNF-α(10 ng/mL) for 8 days. The morphological features were observed by inverted microscope. CD11c, CD80, CD83, CDD86 expressions were assayed by flow cytometry. The concentration of IL-12 was measured by ELISA. The antigen presenting function of the cells were tested by mixed lymphocyte reaction(MLR). Electrochemical measurement was used for the detection of glucose metabolism. Results The typical DC morphological features were not observed from the cells in ALL group. The cells in ALL group expressed lower levels of CD11c, CD83, CD80 and CD86 than the cells in control group(P<0.05). The cells in ALL group secreted lower level of IL-12 than control group(P<0.05). The cells in ALL group displayed weaker activity of stimulating T lymphocyte proliferation than control group in MLR test. Glucose concentration in culture medium of ALL group was higher than that of control group(P<0.05). Conclusion DC derived from B-ALL patients displays a less mature phenotype with compromised functions. Low glucose metabolism ability might be a possible reason for the DC defects in ALL.

Key words: Glucose metabolisom, Leukemia, acute, Lymphoblastic, Dendritic cells

中图分类号:

R725.5

庄泳, 李栋, 付金秋, 时庆, 鞠秀丽. 儿童B系急性淋巴细胞白血病树突状细胞的生物学特性[J]. 山东大学学报（医学版）, 2014, 52(11): 60-64.

ZHUANG Yong, LI Dong, FU Jinqiu, SHI Qing, JU Xiuli. Biological features of dendritic cells derived from childhood B lineage acute lymphoblastic leukemia in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(11): 60-64.

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儿童B系急性淋巴细胞白血病树突状细胞的生物学特性

Biological features of dendritic cells derived from childhood B lineage acute lymphoblastic leukemia in vitro

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