1H-MRS在检测胶质瘤早期放疗反应中的应用

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (4): 51-55.

1H-MRS在检测胶质瘤早期放疗反应中的应用

孙珊珊1，刘宏1，董文艳1，姜政2，王建波1，谭炳煦1，王庆伟1

山东大学齐鲁医院 1.肿瘤中心放疗科； 2.神经外科，济南 250012

收稿日期:2012-01-21 出版日期:2012-04-10 发布日期:2012-04-10
通讯作者: 王庆伟(1965- ),女，教授，硕士生导师，主要从事肿瘤放射治疗学的基础及临床研究。 E-mail:wangqw@sdu.edu.cn
作者简介:孙珊珊(1986- )，女，硕士研究生，主要从事肿瘤放射治疗学研究。
基金资助:
山东省科技发展计划项目(2007GG30002017)

Application of 1H-MRS in detecting early
radiotherapy response in glioma

SUN Shan-shan1， LIU Hong1， DONG Wen-yan1， JIANG Zheng2，
WANG Jian-bo1， TAN Bing-xu1, WANG Qing-wei1

1. Department of Radiotherapy of Oncology Center； 2. Department of Neurosurgery，
Qilu Hospital of Shandong University, Jinan 250012

Received:2012-01-21 Online:2012-04-10 Published:2012-04-10

摘要/Abstract

摘要：

目的观察大鼠C6胶质瘤模型放疗前后氢质子磁共振波谱(1H-MRS)表现,探讨其在早期监测肿瘤治疗反应中的作用。方法建立SD大鼠C6胶质瘤模型，分为照射组(n=10)和未照射组(n=12)，建模后第9天给予照射组荷瘤鼠单次大剂量外照射(15Gy/次/野)。两组大鼠建模后均接受1H-MRS扫描，选择N-乙酰天冬氨酸(NAA)、胆碱(Cho)、乳酸(Lac)为检测肿瘤组织代谢的指标，同时行磁共振扫描(MRI)检测肿瘤体积的变化；连续观察建模后两组代谢物及体积变化，并对两组代谢指标变化与肿瘤体积变化行相关性分析。结果所有大鼠均成功种植肿瘤；未照射组随肿瘤体积增大，Cho、Lac浓度增加，NAA浓度减小，代谢物变化与体积变化呈显著相关性(Cho: r=0.958, P＜0.001；Lac: r=0.989, P＜0.001；NAA: r=－0.943, P＜0.001)；照射组放疗后1d，Cho由3.37mmol/kg降至2.0mmol/kg(P＜0.01)。放疗后3d，Lac由2.34mmol/kg降至1.59mmol/kg(P＜0.01),NAA较治疗前无显著改变(P＞0.05)，放疗后3d肿瘤体积较治疗前增加31.4%(P＜0.01)，放疗后5d体积开始减小，随后出现肿瘤生长抑制，各代谢指标继续下降且与体积变化呈相关趋势(Cho: r=0.696, P=0.304；Lac: r=0.911, P=0.089；NAA: r=－0.583, P=0.417)，MRI检测的体积发生变化之前，1HMRS检测的代谢物已经出现抑制性变化。结论 1H-MRS可无创性获得胶质瘤放疗前后代谢物NAA、Cho、Lac变化信息，较早监测早期治疗反应，敏感性优于MRI，是一种有效的判断近期疗效的检查方法。

关键词: C6胶质瘤模型；1H-MRS；放射疗法;SD大鼠

Abstract:

Objective To observe the 1H-MRS of C6 glioma models before and after radiotherapy, and to discuss its effect in detecting early response in oncotherapy. Methods C6 glioma models of SD rats(treated group, n=10； untreated group, n=12)were applied and a single dose of 15Gy was given to the treated group 9 days after tumor implantation. After tumor implantation, 1H-MRS was used to detect changes in tumor metabolism, such as N-Acetyl-L-aspartic acid (NAA), Choline (Cho) and Lactic acid (Lac). MRI was used to detect changes of tumor volume. Changes of metabolism and volume in the two groups were observed and correlation analysis was done for indices changes and tumor size changes of the two groups. Results Rate of tumor formation was 100% in all rats. In the untreated group (n=12), Cho and Lac showed an ascendant trend and NAA gradually decreased with tumor growth. Changes of tumor metabolites and tumor volume had significant correlations (Cho: r=0.958, P＜0.001；Lac: r=0.989, P＜0.001；NAA: r=－0.943, P＜0.001). In the treated group(n=10), Cho decreased from 3.37mmol/kg to 2.0mmol/kg(P＜0.01) 1 day after radiation, Lac decreased from 2.34mmol/kg to 1.59mmol/kg(P＜0.01) 3 days after radiation and there was no significant change in NAA(P＞0.05). Tumor volume increased 31.4%(P＜0.01) at 3rd day and began to shrink at 5th day after radiation, and then tumor growth inhibition appeared , the metabolic indices continued to fall and there was a correlation trend between tumor volume changes and metabolic indices (Cho: r=0.696, P=0.304; Lac: r=0.911, P＜0.089；NAA: r=－0.583, P＜0.417). Before volume changes detected by MRI began, inhibitive changes in metabolites detected by 1H-MRS had already happened. Conclusion 1H-MRS can detect metabolism changes of glioma such as NAA, Cho and Lac before and after noninvasive radiotherapy. It is more sensitive in monitoring early response to radiation than MRI and is an effective method in determining the short-term effect of treatments.

Key words: C6 glioma model; 1H -MRS; Radiotherapy; SD rats

中图分类号:

R73-3

孙珊珊1，刘宏1，董文艳1，姜政2，王建波1，谭炳煦1，王庆伟1. 1H-MRS在检测胶质瘤早期放疗反应中的应用[J]. 山东大学学报(医学版), 2012, 50(4): 51-55.

SUN Shan-shan1， LIU Hong1， DONG Wen-yan1， JIANG Zheng2， WANG Jian-bo1， TAN Bing-xu1, WANG Qing-wei1. Application of 1H-MRS in detecting early
radiotherapy response in glioma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(4): 51-55.

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