雷洛昔芬对大鼠垂体瘤GH3细胞Seladin-1基因表达与增殖的影响

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (4): 47-50.

雷洛昔芬对大鼠垂体瘤GH3细胞Seladin-1基因表达与增殖的影响

李彦斌，滕良珠，吴洪喜，张永超，辛涛，郑志明，张振

山东大学附属省立医院神经外科，济南 250021

收稿日期:2011-12-09 出版日期:2012-04-10 发布日期:2012-04-10
通讯作者: 滕良珠(1952- )，男，教授，博士生导师，主要从事颅内肿瘤及颅脑外伤的研究工作。 E-mail：tenglz@126.com
作者简介:李彦斌(1979- )，男，硕士研究生，主治医师，主要从事颅内肿瘤的研究工作。
基金资助:
山东省科技攻关计划项目(2010GGB14010)；国家自然科学基金资助项目(81100836)

Effects of raloxifene on proliferation and seladin-1
gene expression in GH3 cells

LI Yan-bin, TENG Liang-zhu, WU Hong-xi, ZHANG Yong-chao,
XIN Tao, ZHENG Zhi-ming, ZHANG Zhen

Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2011-12-09 Online:2012-04-10 Published:2012-04-10

摘要/Abstract

摘要：

目的探讨选择性雌激素受体拮抗剂雷洛昔芬对大鼠垂体瘤GH3细胞株的seladin-1基因表达及其对瘤细胞增殖的影响。方法以不同浓度(0.1～1000nmol/L)的雷洛昔芬作用于大鼠GH3细胞，培养48h后检测雷洛昔芬的作用效果；荧光定量RT-PCR和Western blot分别检测seladin-1mRNA和蛋白；Cell Counting Kit-8试剂盒检测不同浓度药物对瘤细胞增殖的影响。结果雷洛昔芬在10～100nmol/L的浓度范围能有效抑制selandin-1的表达，瘤细胞增殖受抑制(P<0.05)。结论雷洛昔芬能够有效抑制大鼠垂体瘤GH3细胞株selandin-1表达及瘤细胞生长，可作为垂体瘤的药物和基因治疗的新靶点。

关键词: 雷洛昔芬；seladin-1；GH3细胞；垂体瘤

Abstract:

Objective To explore the effects of raloxifene, a selective estrogen receptor antagonist, on proliferation and seladin-1 gene expression in rat GHsecreting(GH3)cells. Methods 48h after raloxifene was added in a series of concentrations(0.1～1000nmol/L)to GH3 cells, the expression level of the seladin-1 gene was examined by quantitative real time RT-PCR and Western blot , and cell proliferation was investigated using the Cell Counting Kit-8. Results With quantitative real time RT-PCR and Western blot, it was found that, with the drug within the 10-100nmol/L range, seladin-1 expression was significantly down-regulated and cell proliferation was inhibited in a concentration-dependent manner (P<0.05). Conclusion Raloxifene suppresses seladin-1 expression and GH3 cell proliferation and, hence, it holds the promise of being used in treating pituitary adenoma. The seladin-1 gene may be a new target for gene therapy.

Key words: Raloxifene; Seladin-1; GH3 cells; Pituitary adenoma

中图分类号:

R736.4

李彦斌，滕良珠，吴洪喜，张永超，辛涛，郑志明，张振. 雷洛昔芬对大鼠垂体瘤GH3细胞Seladin-1基因表达与增殖的影响[J]. 山东大学学报(医学版), 2012, 50(4): 47-50.

LI Yan-bin, TENG Liang-zhu, WU Hong-xi, ZHANG Yong-chao, XIN Tao, ZHENG Zhi-ming, ZHANG Zhen. Effects of raloxifene on proliferation and seladin-1
gene expression in GH3 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(4): 47-50.

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