慢性铝中毒对大鼠海马CA3区神经胶质细胞的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (6): 20-23.

慢性铝中毒对大鼠海马CA3区神经胶质细胞的影响

蒋鸫，康朝胜，杨向斌

贵阳医学院，基础医学院解剖学教研室，贵阳 550004

收稿日期:2011-01-05 出版日期:2011-06-10 发布日期:2011-06-10
通讯作者: 康朝胜(1965- )，男，副教授，硕导，主要从事重金属对中枢神经影响的研究。 E-mail:gykcs163.com
作者简介:蒋鸫 (1977- )，女，讲师，硕士，主要从事重金属对中枢神经影响的研究。 E-mail:jiangdong770126@yahoo.com.cn
基金资助:
贵州省教育厅自然科学基金资助项目(黔教科2007-031)。

Effects of chronic aluminum toxicity on neuroglial cells in the hippocampal CA3 area of rats

JIANG Dong, KANG Chao-sheng, YANG Xiang-bin

Department of Anatomy, Basic Medical College, Guiyang Medical University, Guiyang 550004, China

Received:2011-01-05 Online:2011-06-10 Published:2011-06-10

摘要/Abstract

摘要：

目的观察慢性铝中毒对大鼠海马CA3区神经胶质细胞的影响，探讨铝的神经毒性。方法随机将4～5月龄SD大鼠60只分为铝中毒组和正常对照组各30只，雌雄各半。常规石蜡切片，经HE染色定位，胶质纤维酸性蛋白(GFAP)、CD11b、半乳糖脑苷脂(GC)免疫组化染色，对海马CA3区GFAP、CD11b、GC阳性反应细胞计数，用细胞形态学计量方法测量GFAP、CD11b、GC阳性反应产物的平均光密度值，透射电镜观察海马CA3区神经胶质细胞超微结构的变化。结果与正常对照组比较, 铝中毒组大鼠GFAP、CD11b阳性细胞数量增多，阳性产物平均光密度值增高；GC阳性细胞数量减少, 阳性产物平均光密度值降低；电镜下神经胶质细胞水肿，线粒体减少，嵴断裂，胶质丝模糊。结论慢性铝中毒可导致大鼠海马CA3区星型胶质细胞和小胶质细胞反应性增多，而少突胶质细胞数量减少，神经胶质细胞超微结构改变。

关键词: 铝中毒；海马；神经胶质细胞

Abstract:

Objective To observe the effect of chronic aluminum toxicity on glial cells in the hippocampal CA3 area of rats, and to investigate the neurotoxicity of aluminum. Methods 60 4-5-month old Sprague-Dawley rats were randomly divided into the aluminum exposed group and the normal control group, 15 males and 15 females in each group. After paraffin section and location with HE staining, immunohistochemistry staining of glial fibrillary acidic protein(GFAP), CD11b and galactose cerebroside(GC) was done. The GFAP, CD11b- and GC-positive cells in the hippocampal CA3 area were quantitatively analyzed by the cell morphometric technique. The ultrastructure of neuroglial cells was observed by an electron microscopy. Results Compared with the normal control group, numbers and average optical densities of GFAP and CD11bpositive cells in the hippocampal CA3 area were significantly increased, while the number and average optical density of GC-positive cells were significantly decreased in the aluminum exposed group. Under an electron microscope, glial cells swelled, the number of mitochondria decreased, crista broke and glial filaments were vague in the aluminum exposed group. Conclusion Chronic aluminum toxicity can increase numbers of astrocytes and microglia, decrease the number of oligodendrocytes in the hippocampal CA3 area, and change the ultrastructure of glial cells.

Key words: Aluminum poisoning; Hippocampus; Neuroglial cells

中图分类号:

R338.1

蒋鸫，康朝胜，杨向斌 . 慢性铝中毒对大鼠海马CA3区神经胶质细胞的影响[J]. 山东大学学报(医学版), 2011, 49(6): 20-23.

JIANG Dong, KANG Chao-sheng, YANG Xiang-bin. Effects of chronic aluminum toxicity on neuroglial cells in the hippocampal CA3 area of rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 20-23.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	朱俊德,余资江,戈果. noggin对帕金森病大鼠黑质神经元的保护作用[J]. 山东大学学报(医学版), 2010, 48(2): 52-57.
[2]	. Noggin蛋白对D半乳糖致衰老小鼠学习记忆及海马齿状回神经发生的影响[J]. 山东大学学报(医学版), 2009, 47(8): 72-74.