Egb761抑制谷氨酸受体拮抗大鼠海马神经元兴奋毒损伤

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (6): 15-19.

Egb761抑制谷氨酸受体拮抗大鼠海马神经元兴奋毒损伤

刘永1，顾颖慧1，彭艳1，周国平2，周登明2，苗蓓3，侯筱宇1

1. 徐州医学院生物化学与分子生物学研究中心，江苏省脑病生物信息重点实验室, 江苏徐州221002；
2. 徐州医学院临床医学系, 江苏徐州 221002；
3. 江苏省麻醉学重点实验室, 江苏徐州221002

收稿日期:2010-11-29 出版日期:2011-06-10 发布日期:2011-06-10
作者简介:刘永(1974- )，男，博士，副教授，主要从事细胞信号转导研究。E-mail: yliuphd@163.com
基金资助:
江苏省“青蓝工程”资助项目(2008)；徐州医学院大学生实践创新训练计划资助项目(Xdc007-14)；徐州医学院基础学院大学生实践创新训练计划资助项目(2010)；江苏高校优势学科建筑工程资助项目。

Neur-oprotection of Egb761 against glutamate excitotoxicity through inhibiting glutamate receptors in cultured rat hippocampal neurons

LIU Yong1, GU Ying-Hui1, PENG Yan1, ZHOU Guo-ping2, ZHOU Deng-ming2, MIAO Bei3, HOU Xiao-yu1

1. Research Center for Biochemistry & Molecular Biology, Jiangsu Key Laboratory of Brain Disease Bioinformation;
2. Department of Clinical Medicine; 3. Jiangsu Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221002, Jiangsu, China

Received:2010-11-29 Online:2011-06-10 Published:2011-06-10

摘要/Abstract

摘要：

目的观察银杏叶提取物Egb761对海马神经元谷氨酸(Glu)兴奋毒性损伤的保护作用及其机制。方法采用DAPI染色和TUNEL法检测Glu诱导的海马神经元细胞凋亡及Egb761的保护作用，采用全细胞膜片钳技术记录Egb761对大鼠海马神经元Glu受体电流的抑制作用。结果 Egb761拮抗Glu孵育诱导的海马神经元凋亡样死亡，其分子机制可能是抑制N甲基-D-天冬氨酸(NMDA)型和海人酸(KA)型Glu受体。结论银杏叶提取物Egb761通过抑制Glu离子通道而拮抗Glu对海马神经元的兴奋毒作用。

关键词: Egb761；受体，N-甲基-D-天冬氨酸；受体，KA；兴奋毒；膜片钳术

Abstract:

Objective To study the mechanism of neuro-protection of ginkgo biloba leaf extract Egb761 against glutamate excito-toxicity. MethodsEffects of Egb761 on glutamate-induced apoptosis-like neuronal cell death were investigated by DAPI staining and TUNEL. Effects of Egb761 on glutamate-induced whole-cell currents were detected by patch-clamp recording in cultured hippocampal neurons. Results Egb761 could inhibit glutamate-induced apoptosis-like cell death in cultured rat hippocampal neurons. Egb761 could also dose-dependently inhibit glutamate-induced whole-cell currents, including NMDA receptors and kainate acid(KA) receptors. Conclusion Egb761 could protect cultured hippocampal neurons against glutamate excitotoxicity through blocking iontrophic glutamate receptors.

Key words: Egb761; Receptors, N-methyl-D-aspartate; Receptors, kainate acid; Excitotoxicity; Patch-clamp techniques

中图分类号:

Q593+.2

刘永1，顾颖慧1，彭艳1，周国平2，周登明2，苗蓓3，侯筱宇1. Egb761抑制谷氨酸受体拮抗大鼠海马神经元兴奋毒损伤[J]. 山东大学学报(医学版), 2011, 49(6): 15-19.

LIU Yong1, GU Ying-Hui1, PENG Yan1, ZHOU Guo-ping2, ZHOU Deng-ming2, MIAO Bei3, HOU Xiao-yu1. Neur-oprotection of Egb761 against glutamate excitotoxicity through inhibiting glutamate receptors in cultured rat hippocampal neurons[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 15-19.

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