新型生物活性复合材料RGD-PLGA/HA体外细胞相容性的实验研究

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (5): 29-33.

新型生物活性复合材料RGD-PLGA/HA体外细胞相容性的实验研究

张士杰1，陈飞1，蒋欣泉2

1.山东大学第二医院口腔正畸科, 济南 200033; 2.上海交通大学医学院附属第九人民医院口腔颌面外科，上海 200072

收稿日期:2010-07-30 出版日期:2011-05-10 发布日期:2011-05-10
作者简介:张士杰（1966- ），男，副教授，硕士生导师，主要从事牙齿矫正方面的研究。 E-mail: meijie0909@sina.com
基金资助:
山东省自然科学基金资助项目（Y2008C66 ）；山东省科技攻关项目（2009GG10002059）

Cell compatibility of a new type polyporous RGD-PLGA/HA scaffold in vitro

ZHANG Shi-jie1， CHEN Fei1， JIANG XIN-quan2

1. Department of Orthodontics, Shandong University of the second hospital, Jinan 250033, China;
2. Department of Oral and Maxillofaeial Surgery, College of Stomatology, Ninth People′s Hospital, Shanghai Jiao Tong University, Shanghai 200072, China

Received:2010-07-30 Online:2011-05-10 Published:2011-05-10

摘要/Abstract

摘要：

目的研究新型多孔多肽聚乳酸乙醇酸/羟基磷灰石(RGD-PLGA/HA)支架材料的体外细胞相容性。方法采用贴壁法对兔骨髓基质细胞(BMSCs)进行体外矿化诱导培养，扩增后与实验A组（RGD-PLGA/HA）、实验B组（PLGA/HA）分别进行体外复合培养，并通过定性及定量法检测BMSCs在材料表面的黏附能力、增殖活力，验证细胞材料复合体的成骨活性。结果兔BMSCs在每组材料的表面均能生长，经体外诱导后在支架材料的表面形成钙结节，实验A组细胞的黏附及增殖能力均强于实验B组。结论兔BMSCs与新型多孔RGD-PLGA/HA支架材料有良好的相容性。

关键词: 骨髓基质细胞；多肽聚乳酸乙醇酸/羟基磷灰石支架；组织工程;兔

Abstract:

Objective To study the cell biocompatibility of a new type polyporous RGD-PLGA/HA scaffold in vitro. Methods Bone marrow stromal cells (BMSCs) obtained from rabbits were cultured, induced and proliferated in vitro. The cells were incubated with RGD-PLGA/HA in experimental group A and PLGA/HA in group B. The capacity of adhesion and proliferation of BMSCs were qualitatively and quantitatively examined, and the osteogenesis activity of cell composite was tested. Differences between the 2 groups were analyzed. Results BMSCs of rabbits were capable of growth in both groups. After the induction in vitro, a calcium nodule formed on the surface of the scaffold material, and the capacity of adhesion and proliferation were stronger in group A than in control group B(P＜0.05). Conclusion Polyporous RGD-PLGA/HA as a scaffold for bone tissue engineering shows good biocompatibility with BMSCs.

Key words: BMSCs； RGD-PLGA/HA scaffold； Tissue engineering; Rabbits

中图分类号:

R318.08

张士杰1，陈飞1，蒋欣泉2. 新型生物活性复合材料RGD-PLGA/HA体外细胞相容性的实验研究[J]. 山东大学学报(医学版), 2011, 49(5): 29-33.

ZHANG Shi-jie1，CHEN Fei1，JIANG XIN-quan2. Cell compatibility of a new type polyporous RGD-PLGA/HA scaffold in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(5): 29-33.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	陈善良1,许莉2，王雪梅2，刘羽曦3，厉泉2，张辉2，路平2，刘天起2. 戊二醛鞣制驴心包与牛心包的体外力学性能比较[J]. 山东大学学报(医学版), 2012, 50(6): 42-.
[2]	王建光1，刘天起2，王雪梅2，许莉2，刘羽曦3，王东2，厉泉2. 京尼平与戊二醛鞣制牛心包材料的对比研究[J]. 山东大学学报(医学版), 2011, 49(5): 24-28.