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山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (5): 24-28.

• 论文 • 上一篇    下一篇

京尼平与戊二醛鞣制牛心包材料的对比研究

王建光1,刘天起2,王雪梅2,许莉2,刘羽曦3,王东2,厉泉2   

  1. 1.潍坊医学院, 山东 潍坊 261053; 2.山东大学附属千佛山医院心外科, 济南 250014;3.山东中医药大学, 济南 250014
  • 收稿日期:2011-02-18 出版日期:2011-05-10 发布日期:2011-05-10
  • 通讯作者: 刘天起(1959- ),男,教授,硕士生导师,主要从事心脏移植及生物瓣膜研究。 E-mail: tianqiliu@hotmail.com
  • 作者简介:王建光(1982- ),男,硕士研究生,主要从事生物瓣膜研究。 E-mail:wangjianguang04272@163.com
  • 基金资助:

    山东省科技攻关计划资助项目(2007GG10002003)

Comparison of genipin- and glutaraldehyde-tanned bovine pericardia

WANG Jian-guang1, LIU Tian-qi2,  WANG Xue-mei2, XU Li2, LIU Yu-xi3, WANG Dong2,  LI Quan2    

  1. 1. Weifang Medical University, Weifang 261053, Shandong,  China; 2. Department of Cardiac Surgery, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
    3. Shandong University of Traditional Chinese Medicine, Jinan 250014,  China
  • Received:2011-02-18 Online:2011-05-10 Published:2011-05-10

摘要:

目的     观察京尼平作为一种新型固定生物瓣膜组织交联剂的可行性。方法     牛心包经脱细胞处理,分别用戊二醛和京尼平进行鞣制,光镜下观察脱细胞效果,电镜下观察胶原纤维排列;测试收缩温度;拉伸试验测定最大载荷;Wistar大鼠臀大肌皮下埋藏牛心包片,于4周及8周后取出,分别测试京尼平与戊二醛鞣制的牛心包的钙、磷含量。结果    经脱细胞处理后,光镜下可见脱细胞处理的牛心包几乎没有细胞核,而未经脱细胞处理的牛心包可见弥漫存在的细胞核。电镜下可见京尼平鞣制的牛心包胶原纤维呈环状交联,戊二醛鞣制的牛心包胶原纤维呈网状交联,京尼平鞣制的牛心包胶原纤维排列较戊二醛鞣制的牛心包略为疏松,收缩温度略低于戊二醛鞣制的牛心包(P<0.05)。最大载荷无统计学差异(P>0.05)。但京尼平鞣制牛心包的钙、磷含量显著低于戊二醛鞣制的牛心包 (P<0.05)。京尼平鞣制的牛心包8周的钙含量显著低于4周的钙含量(P<0.05)。结论     京尼平鞣制的牛心包具有较高的强度和极低的钙、磷含量,且随时间延长京尼平鞣制的牛心包的钙含量有减少趋势,提示京尼平是一种很有前景的生物组织交联试剂。

关键词: 京尼平;戊二醛;牛心包;鞣制;抗钙化

Abstract:

Objective      To evaluate the feasibility of genipin as a new biological valve cross-linking agent. Methods     Acellular bovine pericardia were respectively treated with  glutaradehyde and genipin for tanning. The HE slides of bovine pericardial were observed under a light microscope to identify the decellularized effect. Collagen fiber arrangements were observed under an electron microscope. Shrinkage temperature and tensile strength of the biological materials were tested. Bovine pericardia were buried in the subcutaneous of the musculus glutaeus maximus of Wistar rats for 4 weeks or 8 weeks, and then calcium and phosphorus contents were determined in them. Results    In decellularized bovine pericardial slides, there was almost no nucleus, while in non-decellularized ones, there were many nuclei. Collagen fibers formed a ring in genipin-tanned bovine pericardial tissues, while they were reticulated in glutaraldehyde-tanned tissues. Collagen fiber arrangements were a little looser in genipin tanning than in glutaraldehyde tanning. The shrinkage temperature of genipin-tanned tissues was lower than that of glutaraldehydetanned ones(P<0.05). The difference had no statistical significance in maximum tensile strength between genipin tanning and glutaraldehyde tanning(P>0.05). Calcium and phosphorus contents in genipin tanning were significantly lower than those in glutaraldehyde tanning at 4 weeks and 8 weeks (P<0.05). The calcium content in genipin tanning was significantly lower at 8 weeks than at 4 weeks(P<0.05).  Conclusion     Calcium and phosphorus contents of genipin-tanned bovine pericardial tissues are significantly lower than those of glutaraldehyde-tanned ones, and the calcium content in genipintanned bovine pericardial tissues decreased with time. Tensile strength is higher in genipin-tanned bovine pericardial tissues. Consequently, genipin is a promising crosslinking agent for biological tissues.

Key words: Genipin; Glutaraldehyde; Bovine pericardium; Tanning; Anticalcification

中图分类号: 

  • R318.08
[1] 陈善良1,许莉2,王雪梅2,刘羽曦3,厉泉2,张辉2,路平2,刘天起2. 戊二醛鞣制驴心包与牛心包的体外力学性能比较[J]. 山东大学学报(医学版), 2012, 50(6): 42-.
[2] 张士杰1,陈飞1,蒋欣泉2. 新型生物活性复合材料RGD-PLGA/HA体外细胞相容性的实验研究[J]. 山东大学学报(医学版), 2011, 49(5): 29-33.
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