活化增视颗粒对实验性PVR中MCP-1和TGF-β2的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (4): 61-64.

活化增视颗粒对实验性PVR中MCP-1和TGF-β2的影响

郝延蕾1,满晓飞2,李志红1, 原越3,于海群1

1.山东大学医学院，济南 250012； 2.山东大学附属济南市中心医院眼科，济南 250013;
3.潍坊医学院, 山东潍坊 261053

收稿日期:2010-07-27 出版日期:2011-04-10 发布日期:2011-04-10
通讯作者: 李志红（1957- ），女，教授，硕士生导师，主要从事眼底病学方向研究。 E-mail:lizhihong1957827@163.com
作者简介:郝延蕾（1985- ），女，硕士研究生，主要从事眼底病学方向研究。
基金资助:
山东省自然科学基金资助项目（Y2007C129）。

Effects of huohuazengshi medicinalgranules on MCP-1 and TGF-β2 in experimental proliferative vitreoretinopathy

HAO Yan-lei1， MAN Xiao-fei2， LI Zhi-hong1， YUAN Yue3， YU Hai-qun1

1. School of Medicine, Shandong University, Jinan 250012, China;
2. Department of Ophthalmology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
3. Weifang Medical College, Weifang 261053, Shandong, China

Received:2010-07-27 Online:2011-04-10 Published:2011-04-10

摘要/Abstract

摘要：

目的通过研究活化增视颗粒对兔眼实验性增殖性玻璃体视网膜病变(PVR)玻璃体中MCP-1以及TGF-β2的影响，探讨活化增视颗粒对PVR的作用机制。方法将45只新西兰大白兔随机平均分为模型对照组、沃丽汀组、活化增视颗粒组，采用兔眼玻璃体内注射巨噬细胞的方法建立PVR的动物模型, 各组于3、7、14、21、28d分别随机处死3只，ELISA检测玻璃体液中MCP-1和TGF-β2的质量浓度。结果活化增视颗粒组在7d时可显著降低兔玻璃体内MCP-1表达, 与沃丽汀组及对照组相比差异有统计学意义（P<0.01）,活化增视颗粒组在3d时玻璃体内TGF-β2质量浓度开始升高,与对照组相比差异有统计学意义（P<0.01）, 与沃丽汀组相比差异无统计学意义（P>0.05），至14d时达到高峰，与对照组及沃丽汀组相比差异有统计学意义（P<0.01)。结论活化增视颗粒主要在PVR炎症期及增生早期通过降低玻璃体中MCP1和升高玻璃体中TGFβ2的质量浓度来抑制PVR的发生。

关键词: 增殖性玻璃体视网膜病变（PVR）；活化增视颗粒； MCP-1；TGF-β2

Abstract:

Objective To study the curative and preventive effects and the mechanism of huohuazengshi(HHZS) medicinalgranules on proliferative vitreoretinopathy(PVR) through establishing an experimental animal model of PVR. Methods 45 rabbits (45 eyes) were randomly divided into the model group, the joletion tablet group and the HHZS medicinalgranules group. Experimental PVR models were induced by injecting macrophages into the vitreous. Contents of MCP-1 and TGF-β2 in the vitreous humour were determined by ELISA. Then the rabbits were executed at days 3, 7, 14, 21 and 28. Results Compared with the model group and the joletion tablet group, the content of MCP-1 in the HHZS medicinalgranules group was significantly decreased at day 7(P<0.01), while the content of TGF-β2 was increased at day 3 and reached the peak at day 14(P<0.01). Conclusion HHZS medicinalgranules could inhibit the formation and development of PVR by decreasing the content of MCP-1 and increasing the content of TGF-β2 in the vitreous at the inflammatory phase and early proliferative phase.

Key words: Proliferative vitreoretinopathy; Huohuazengshi medicinalgranules; MCP-1; TGF-β2

中图分类号:

R776.4

郝延蕾1,满晓飞2,李志红1, 原越3,于海群1. 活化增视颗粒对实验性PVR中MCP-1和TGF-β2的影响[J]. 山东大学学报(医学版), 2011, 49(4): 61-64.

HAO Yan-lei1， MAN Xiao-fei2， LI Zhi-hong1， YUAN Yue3， YU Hai-qun1. Effects of huohuazengshi medicinalgranules on MCP-1 and TGF-β2 in experimental proliferative vitreoretinopathy[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 61-64.

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