川续断和丹参影响大鼠正畸牙移动的比较

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (8): 60-64.

川续断和丹参影响大鼠正畸牙移动的比较

梅银生1，2，贾玉龙1，张丽娜1，靳淑梅1，张君1，3

1.山东大学口腔医学院正畸科，济南 250012； 2.武警山东总队医院口腔科，济南 250014；
3.山东省口腔生物医学重点实验室，济南 250012

收稿日期:2010-03-10 出版日期:2010-08-16 发布日期:2010-08-16
通讯作者: 张君（1962- ），男，教授，硕士生导师，主要从事口腔正畸临床及研究。 E-mail：zhangj@sdu.edu.cn
作者简介:梅银生（1980- ），男，住院医师，主要从事口腔正畸临床及研究。
基金资助:
山东省科学技术发展计划资助项目（2008GG2NS02013）；山东省自然科学基金资助项目（Q2008C12）

Effect of Dipsacaceae and Salvia on orthodontic teeth movement

MEI Yinsheng1，2, JIA Yulong1， ZHANG Lina1， JIN Shumei1， ZHANG Jun1，3

1. Department of Orthodontics, School of Stomatology, Shandong University, Jinan 250012, China；
2. Department of Stomatology, Shandong Crops Hospital of Chinese People′s Armed Police Force, Jinan 250014, China；
3. Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China

Received:2010-03-10 Online:2010-08-16 Published:2010-08-16

摘要/Abstract

摘要：

目的研究中药川续断和丹参在大鼠正畸牙移动过程中对其牙周组织改建的作用，并比较两种不同中药在改建过程中的异同。方法选取72只8周龄SPF级Wistar雌性大鼠，建立大鼠正畸牙移动实验模型，随机分为川续断组、丹参组和对照组3组，每组24只，川续断组每日灌服6g/kg川续断水煎剂，丹参组每日灌服6g/kg丹参水煎剂，对照组每日灌服3mL生理盐水，每隔7d加力1次。3组动物于正畸加力7、14、21、28d分批次处死，每批次6只。剥离头颅骨，分别测量牙移动的距离及牙槽骨的密度，同时制作上颌第一磨牙区牙周组织切片，光学、电子显微镜观察牙周组织改建情况，并进行统计学分析。结果川续断组、丹参组牙移动距离差异无统计学意义（P＞0.05），但是二者均大于对照组，差异有统计学意义（P＜0.05）；光镜下观察3组破骨细胞数均呈现先增加后平缓趋势，川续断组、丹参组比对照组增加更为显著（P＜0.05）,川续断组和丹参组之间差异无统计学意义（P＞0.05）。3组牙槽骨密度呈现降低趋势，川续断组、丹参组比对照组降低缓慢（P＜0.05），川续断组和丹参组之间差异无统计学意义（P＞0.05）。结论灌服丹参和川续断水煎剂均促进大鼠正畸牙移动过程中破骨细胞的增殖和分化，加快牙槽骨吸收及其修复重建，有利于正畸牙移动。

关键词: 川续断；丹参；正畸牙移动；大鼠，Wistar

Abstract:

Objective To detect and compare the effect of the traditional Chinese drugs, Dipsacaceae and Salvia, on the reconstruction of paradentium in orthodontic teeth movement. Methods 72 female, 8-week-old SPF Wistar rats were selected and the animal models for orthodontic tooth movement were established. The rats were divided into three groups randomly: Dipsacaceae group, Salvia group and control group. Each group had 24 rats. Dipsacus asperoides decoction(6g/kg), Salvia decoction(6 g/kg) and 3mL NS were drenched daily to the rats in the Dipsacaceae group, Salvia group and control group respectively. Stability of the appliances were checked everyday and the applied force was raised by adjusting the springs every 7 days. The rats were executed in batch on the 7th、14th、21st、28th day by peeling off the skulls. Distances of the teeth movement were measured and slices of the periodontium of the maxillary first molar were observed under optical microscope. Data were analyzed with PASWstatistics18. Results Teeth movement in the Dipsacaceae group and Salvia group were greater than control group（P＜0.05）while no significant difference（P＞0.05）was found between them. Light microscope revealed the number of osteoclasts in all 3 groups increased rapidly at the beginning but flatly later. Osteoclasts in Dipsacaceae and Salvia group showed much more increase than the control （P＜0.05）, however, no significant difference existed between them(P＞0.05). Though alveolar bone density showed the trend of decrease in all 3 groups, Dipsacaceae and Salvia group decreased slowly(P＜0.05) and nosignificance was discovered between the two(P＞0.05). Conclusion Salvia and Dipsacaceae decoction can promote the multiplication and differentiation of the osteoclasts in rats, benefit alveolar bone reconstruction and accelerate orthodontic tooth movement.

Key words: Dipsacaceae; Salvia; Orthodontic tooth movement; Wistar rat

中图分类号:

R783.5

梅银生1，2，贾玉龙1，张丽娜1，靳淑梅1，张君1，3. 川续断和丹参影响大鼠正畸牙移动的比较[J]. 山东大学学报(医学版), 2010, 48(8): 60-64.

MEI Yinsheng1，2, JIA Yulong1， ZHANG Lina1， JIN Shumei1， ZHANG Jun1，3. Effect of Dipsacaceae and Salvia on orthodontic teeth movement[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(8): 60-64.

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