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山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (7): 64-68.

• 论文 • 上一篇    下一篇

TSP-1诱导生长激素腺瘤细胞凋亡的体外研究

杨伟1,鹿向东2,张丽1,姜萌1,韩允1,曲元明1,徐广明1   

  1. 1.山东大学附属省立医院神经外科, 济南 250021; 2.山东省莱芜市人民医院, 山东 莱芜 271100
  • 收稿日期:2009-09-04 出版日期:2010-07-16 发布日期:2010-07-16
  • 通讯作者: 徐广明(1963- ),男,博士,主任医师,主要从事颅内肿瘤研究。 Email:guangmingxu@sdu.edu.cn
  • 作者简介:杨伟(1977- ),男,博士,主治医师,主要从事颅内肿瘤研究。
  • 基金资助:

    山东省优秀中青年科学家科研奖励基金(2007BS03050)资助课题,项目负责人:杨伟

Thrombospondin-1 induces somatotroph adenoma cell apoptosis in vitro

YANG Wei1, LU Xiangdong2, ZHANG Li1, JIANG Meng1, HAN Yun1, QU Yuanming1,XU Guangming1   

  1. 1. Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
     2. The People′s Hospital of Laiwu City, Laiwu  271100, Shandong , China
  • Received:2009-09-04 Online:2010-07-16 Published:2010-07-16

摘要:

目的   探讨TSP-1对生长激素腺瘤细胞生长的影响以及对TGF-β1基因表达的调节作用。方法    本实验采用12例人生长激素腺瘤原代细胞培养进行体外实验研究,通过MTT实验观察TSP-1对细胞生长的影响,并应用流式细胞仪检测TSP-1对生长激素腺瘤细胞的促进凋亡作用;应用RT-PCR方法检测不同浓度TSP-1作用后的TGF-β1基因表达的改变。结果   低浓度(1~5nmol/L)TSP-1在体外作用48h,即可以抑制生长激素腺瘤细胞生长,其作用随剂量和时间的增加而增强;流式细胞术结果显示,TSP1可明显诱导生长激素腺瘤细胞凋亡;与阴性对照组相比较,任何时段低浓度TSP-1对生长激素腺瘤细胞的TGF-β1mRNA表达无明显影响(P>0.05)。结论   TSP-1在体外可以抑制生长激素腺瘤细胞生长,并能诱导细胞凋亡,其作用随剂量和时间的增加而增强;在体外,低中浓度的TSP-1不能调节生长激素腺瘤细胞的TGF-β1基因表达。

关键词: 生长激素腺瘤;原代细胞培养;细胞凋亡;体外研究

Abstract:

Objective   To investigate the effect of TSP-1 on the growth of primary somatotroph adenoma cells and expression of TGF-β1 in somatotroph adenoma cells in vitro. Methods    Human somatotroph adenoma (12 cases) cells were cultured in vitro, and the growth-inhibiting rate was obtained by MTT. Flow cytometry was applied to evaluate the apoptosis rate in the cultured cells, and RT-PCR was applied to determine TGF-β1 mRNA expression in somatotroph adenoma cells after treated with TSP-1. Results   The growth of primary somatotroph adenoma cells was significantly inhibited by TSP-1 with concentration and time dependence. Even low concentration of TSP-1  (1-5nmol/L) inhibited cell growth in vitro after 48h. The data of flow cytometry suggested that TSP-1 induced somatotroph adenoma cell apoptosis in vitro and the effect was positively related with concentrations and time. No significant down-regulation of TGF-β1 mRNA expression was induced by low concentration of TSP-1 at any acting time, compared with the negative control group (P>0.05). Conclusion     TSP-1 could induce apoptosis and inhibit primary somatotroph adenoma cell proliferation in vitro, and the effect might be positively related with concentrations and time. In vitro, TSP-1 in low or medium concentration could not regulate expression of TGF-β1.

Key words:  Somatotroph adenomas; Primary cell culture; Apoptosis; In vitro study

中图分类号: 

  • R736.4
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