您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (3): 44-47.

• 论文 • 上一篇    下一篇

利多卡因对LPS诱导巨噬细胞HMGB1释放及转位的影响

叶婷,类维富,王焕亮,张丽,周长青   

  1. 山东大学齐鲁医院麻醉科, 济南 250012
  • 收稿日期:2009-11-18 出版日期:2010-03-16 发布日期:2010-03-16
  • 通讯作者: 类维富(1956- ),男,教授,硕士生导师,主要从事靶控麻醉以及自体血回输的安全性的研究。
  • 作者简介:叶婷(1984- ),女,硕士研究生,主要从事临床麻醉以及危重症监护的研究。
  • 基金资助:

    山东省自然科学基金资助项目(Y2007C115)

Effects of Lidocaine on the release and translocation of HMGB1 in macrophages induced by lipopolysaccharide

YE Ting, LEI Wei-fu, WANG Huan-liang, ZHANG Li, ZHOU Chang-qing   

  1. Department of Anaesthesia, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2009-11-18 Online:2010-03-16 Published:2010-03-16

摘要:

目的    观察不同浓度利多卡因对脂多糖(LPS)诱导大鼠腹腔巨噬细胞高迁移率蛋白1(HMGB1)释放及转位的影响。方法    取Wistar大鼠腹腔巨噬细胞置12孔板培养2~3d,分为对照组(C组)、LPS组、利多卡因2mg/L+LPS组(Ll+LPS组)、利多卡因20mg/L +LPS组(L2+LPS组)、利多卡因200mg/L +LPS组(L3+LPS组),分别于6、12、24、48h酶联免疫吸附试验(ELISA)测培养液中HMGB1蛋白浓度。免疫细胞化学染色法观察HMGB1在巨噬细胞内的转位情况。结果    HMGB1蛋白的释放在LPS组刺激12h开始增多,24h达高峰。与LPS组相比,3个利多卡因处理组HMGB1的释放均有不同程度的减少,以终浓度在20mg/L时最显著(P<0.05)。同时,免疫细胞化学染色法还观察到利多卡因对HMGB1从细胞核到细胞浆的转位有抑制作用。结论    利多卡因20mg/L可显著抑制LPS诱导大鼠腹腔巨噬细胞HMGB1释放及转位。

关键词: 利多卡因;脂多糖;巨噬细胞;高迁移率族蛋白B1;大鼠,Wistar

Abstract:

Objective    To investigate the effects of Lidocaine on  expression and translocation of the high mobility group box-l(HMGB1) in rat peritoneal macrophages induced by lipopolysaccharide(LPS). MethodsPeritoneal macrophages obtained from Wistar rats and incubated in 12-well tissue culture plates for 2-3 days were divided into 5 groups: the control group, LPS group, Lidocaine 2mg/L +LPS treatment group (L1+LPS treatment group), Lidocaine 20mg/L +LPS treatment group (L2+LPS treatment group) and the Lidocaine 200mg/L +LPS treatment group (L3+LPS treatment group). After 6, 12, 24 and 48h treatment, the concentrations of HMGB1 in the cell culture medium were measured by ELISA. The translocation of HMGB1 in rat peritoneal macrophages was observed by cellular immunochemistry. ResultsAfter rat peritoneal macrophages were stimulated by LPS, the LPS group showed that the release of HMGB1 was increased at 12h and reached the peak at 24h. Compared with the  LPS group, Lidocaine treatment groups decreased in release of HMGB1 in various degrees especially in the 20mg/L+LPS treatment group(P<0.05). Translocation of HMGB1 from the cell nucleus to the cytoplasm in the macrophage was  obviously suppressed. ConclusionLidocaine(20mg/L) obviously inhibits the release and translocation of HMGB1 in  rat peritoneal macrophages induced by LPS.

Key words: Lidocaine; Lipopolysaccharide; Macrophages; High mobility group box-l; Rats, Wistar

中图分类号: 

  • R631
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!