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山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (3): 34-38.

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慢性砷中毒对成年小鼠齿状回超微结构及学习记忆的影响

孙宝飞1,康朝胜1,余资江1,常傲霜2,李玉飞1   

  1. 贵阳医学院 1. 基础医学院解剖学教研室;2. 电镜教研室,  贵阳 550004
  • 收稿日期:2009-12-02 出版日期:2010-03-16 发布日期:2010-03-16
  • 通讯作者: 康朝胜(1964- ),男,硕导,副教授,主要从事神经解剖学的研究。
  • 作者简介:孙宝飞(1984- ),男,硕士研究生,主要从事神经解剖学的研究。
  • 基金资助:

    筑科计合同字第6-13号

Impact of chronic arsenic poisoning on the dentate gyrus ultra-structure and the learning and memory of the adult mouse

SUN Baofei  1, KANG Chaosheng  1, YU Zijiang  1, CHANG Aoshuang  2,LI Yufei  1    

  1. 1. Department of Anatomy, School of Preclinical Medicine; 2. Department of Transmission Electron Microscopy, Guiyang Medical University, Guiyang 550004, China
  • Received:2009-12-02 Online:2010-03-16 Published:2010-03-16

摘要:

目的   观察慢性砷中毒对小鼠海马齿状回超微结构的改变及小鼠学习记忆的影响。方法   选取健康成年昆明小鼠80只,雌雄各半,分为对照组、慢性砷中毒低、中、高剂量组,每组20只,各染砷组分别以蒸馏水、1/5 LD50、1/10 LD50、1/40 LD50A s2O3灌胃,连续3个月,根据其体重变化随时调整用药剂量。灌胃结束后,采用Y-电迷宫检测各组小鼠学习记忆行为,透射电镜观察齿状回细胞超微结构的变化。结果   高剂量砷中毒组学习、记忆的Y-迷宫测试次数明显高于对照组(P<0.05),电镜下观察中毒组齿状回神经元,可见部分神经细胞膜皱缩,胞体减小,核染色质电子密度增高。结论   慢性砷中毒明显损害成年小鼠学习记忆的能力,可能与海马齿状回神经元受损有关。

关键词: 砷中毒;迷宫学习;记忆;齿状回;小鼠

Abstract:

Objective   To study the effects of chronic arsenic poisoning on the  change of the dentate gyrus ultrastructure and  learning and memory in mice. Methods   80 healthy adult Kunming mice, weighting 20-22g, were divided into four groups: the normal control group, the low dose group, the middle dose group and the high dose group (10 males and 10 females in each group), which were fed with distilled water, 1/5 LD50, 1/10 LD50 and 1/40 LD50 AS2O3 for 3 months  respectively, and  the dose was adjusted according to their weight changes. Then the ability of learning and memory was tested by a Y-maze, and the ultra-structure of the dentate gyrus was observed by transmission electron microscopy. Results    The ability of learning and memory of the high dose group was significantly lower than that of the normal control group(P<0.05), and some neurons of the dentate gyrus of each poisoned group showed a shrunken cellular membrane, diminished cell body and increased electron density of nuclear chromatin under transmission electron microscopy. Conclusion    Chronic arsenic poisoning might damage the ability of learning and memory in mice, which may be related with  damaged neurons of the dentate gyrus.

Key words: Arsenic poisoning; Maze learning; Memory; Dentate gyrus; Mice

中图分类号: 

  • R595
[1] 康朝胜,孙宝飞,余资江,李玉飞. 慢性砷中毒对小鼠齿状回GFAP表达的影响[J]. 山东大学学报(医学版), 2011, 49(6): 28-32.
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