慢性HBV活动性感染者外周血淋巴细胞泛素mRNA的表达及临床意义

山东大学学报(医学版)

慢性HBV活动性感染者外周血淋巴细胞
泛素mRNA的表达及临床意义

王晓菊¹，汪明明²，徐皖苏²，赵胜梅³，崔速南²，李晓迎²，刘春华¹

（1. 山东大学医学院，济南 250012； 2. 济南市传染病医院肝病科，济南 250021；
3. 山东大学齐鲁医院检验科，济南 250012）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-16 发布日期:2009-02-16
通讯作者: 汪明明

Expression of peripheral blood lymphocyte ubiquitin mRNA in chronic HBV infection with active inflammation and its clinical significance

WANG Xiaoju¹，　WANG Mingming²，　XU Wansu²,　ZHAO Shengmei³，CUI Sunan²，　LI Xiaoying²，　LIU Chunhua¹

（1. School of Medicine, Shandong University， Jinan 250012， China；
2. Department of Hepapolocy, Jinan Hospital of Infectious Disease, Jinan 250021， China; 3. Department of Laboratory, Qilu Hospital of Shandong University, Jinan 250012， China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-16 Published:2009-02-16
Contact: WANG Mingming

摘要/Abstract

摘要： 目的探讨外周血淋巴细胞泛素mRNA的表达与慢性HBV活动性感染的关系及临床意义。方法96例慢性HBV感染并有炎症活动者，其中慢性乙肝组（40例），活动性肝炎肝硬化组（30例），慢性重型肝炎组（26例），同时设立健康对照组（26例）。采用实时荧光PCR检测外周血淋巴细胞泛素mRNA的表达，以βactin作为内参。组间差异采用单因素方差分析。结果各组患者外周血淋巴细胞泛素mRNA的表达水平均低于对照组（P＝0.00），其中慢性重型肝炎组表达水平最低,活动性肝硬化组次之。结论慢性HBV感染并有炎症活动者均存在外周血淋巴细胞泛素mRNA表达不足，病情越重表达水平越低；外周血淋巴细胞泛素mRNA的低表达可能与疾病的发生发展有关。

关键词: 肝炎病毒,乙型, 淋巴细胞,外周血, 泛素

Abstract: To investigate the expression of peripheral blood lymphocyte ubiquitin mRNA in chronic HBV active infection and to evaluate its clinical significance. MethodsNinetysix patients were enrolled in the study and were divided into 3 groups： the chronic hepatitis B group（40 patients）, the active cirrhosis group(30 patients) and the severe hepatitis group（26 patients）. In addition, 26 healthy persons served as the control group. Ubiquitin mRNA of the peripheral blood lymphocytes and intrareference βactin were determined by realtime PCR. Differences among groups were analyzed by oneway ANOVA. ResultsExpression level of peripheral blood lymphocyte ubiquitin mRNA in all experimental groups was lower than in the healthy control group(P=0.00), and in the severe hepatitis group it was the lowest and in the active cirrhosis group it was the second. ConclusionExpression of the peripheral blood lymphocyte ubiquitin mRNA is abnormal in all patients, and it may be involved in the occurrence and development of chronic HBV active infection. The lower the expression, the severer the disease.

Key words: HBV, Peripheral blood lymphocyte, Ubiquitin

中图分类号:

R512.6

王晓菊1 ，汪明明2 ，徐皖苏2 ，赵胜梅3 ，崔速南2 ，李晓迎2 ，刘春华1
. 慢性HBV活动性感染者外周血淋巴细胞
泛素mRNA的表达及临床意义

[J]. 山东大学学报(医学版), 2009, 47(02): 58-61.

WANG Xiaoju¹，　WANG Mingming²，　XU Wansu²,　ZHAO Shengmei³，CUI Sunan²，　LI Xiaoying²，　LIU Chunhua¹
. Expression of peripheral blood lymphocyte ubiquitin mRNA in chronic HBV infection with active inflammation and its clinical significance[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 58-61.

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